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- 文献和实验
- 技术资料
- 保存条件：
Powder: -20°C, 3 years.In solvent: -80°C, 6 months; -20°C, 1 month.
- 英文名：
Biotin-XX-SSE
- 库存：
货期：1-2天
- 供应商：
MedChemExpress LLC
- CAS号：
194041-66-2
- 规格：
1 mg/5 mg/10 mg
| 规格： | 1 mg | 产品价格： | ￥540.0 |
|---|---|---|---|
| 规格： | 5 mg | 产品价格： | ￥1260.0 |
| 规格： | 10 mg | 产品价格： | ￥1980.0 |
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Sulfo-NHS-LC-LC-Biotin
CAS No. : 194041-66-2
MCE 国际站：Sulfo-NHS-LC-LC-Biotin
产品活性：Sulfo-NHS-LC-LC-Biotin (Biotin-XX-SSE) 是一种生物素试剂，用于标记暴露于完整外泌体外叶的蛋白 (proteins)，在生物素和胺反应连接剂之间包含一个较大的间隔臂。该连接子的大小有助于克服空间位阻，并提高拥挤外泌体表面的标记效率。
研究领域：Others
作用靶点：Fluorescent Dye
In Vitro: Biotinylation of exosome proteins: Two hundred micrograms of intact exosomes were mixed with 10 mM Sulfo-NHS-LC-LC-Biotin at room temperature for 30 min. Four conditions were taken into account during this experiment: (a) an excess of Sulfo-NHS-LC-LC-Biotin was used to favor a complete saturation of exposed lysine residues and potential N-terminus, (b) the presence of the sulfonate group in Sulfo-NHS-LC-LC-Biotin blocks the reagent from penetrating the exosomal membrane, (c) Sulfo-NHS-LC-LC-Biotin has an spacer arm of 30.5 angstroms which improves the biotinylation of proteins in their natural conformation, and (d) amino acids labeled with Sulfo-NHS-LC-LC-Biotin will have an increase in mass of 452 Da. After incubation, the excess of Sulfo-NHS-LC-LC-Biotin was removed using a 10 KDa MWCO filtration device.
Rat aortic endothelial cells (RAEC) were surface modified in suspension with 1 mM Sulfo-NHS-LC-LC-biotin for 10 min, followed by pelleting and resuspension in PBS.
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文献和实验8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody
8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody
表达系统（178-541aa）：CSB-EP745804 ENL1 多克隆抗体货号（兔多抗）： No conjugated：CSB-PA745804LA01ENL HRP conjugated：CSB-PA745804LB01ENL FITC conjugated：CSB-PA745804LC01ENL Biotin conjugated：CSB-PA745804LD01ENL 参考文献： [1] Systems Genomics
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