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文献和实验岛是否发挥功能依旧需要细胞学实验验证,例如用甲基转移酶抑制剂 5aza 进行验证,最主要的是需要通过测序手段来说明 CpG 岛甲基化情况。如果不存在甲基化,即使存在 n 种甲基化位点,那也没用。
Isolation of CpG Islands Using a Methyl-CpG Binding Column
Vertebrate genomes are globally heavily methylated at the sequence CpG, with the exception of short patches of GC-rich DNA of between 1–2 kb in size that are free of methylation, and these are known as CpG islands (see refs
Isolation of CpG Islands From BAC Clones Using a Methyl-CpG Binding Column
Vertebrate genomes are globally heavily methylated at the sequence CpG with the exception of short patches of GC-rich DNA, usually between 1–2 kb in size, which are free of methylation and these are known as CpG islands (see refs
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