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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
零下80~20°C
- 保质期:
Powder: -20°C for 3 years In solvent: -80°C for 2 years
- 英文名:
HPF
- 库存:
10
- 供应商:
TargetMol
- CAS号:
359010-69-8
- 规格:
1 mg/5 mg/500 μg
| 规格: | 1 mg | 产品价格: | ¥5440.0 |
|---|---|---|---|
| 规格: | 5 mg | 产品价格: | ¥22700.0 |
| 规格: | 500 μg | 产品价格: | ¥2900.0 |
Product Introduction
Bioactivity
英文名:HPF
描述:The biology of highly reactive oxygen radical species is of great interest in many biomedical research disciplines, including neurodegeneration, aging, cancer, and infectious diseases.[1] There are a number of fluorescent reagents, such as 2,7-dichlorodihydrofluorescein (DCDHF), that can be used to detect free radicals, but they have significant limitations due to their facile oxidation by light and numerous non-radical oxidants such as hydrogen peroxide (H2O2). [2] HPF is a cell-permeable aromatic amino-fluorescein derivative that has little intrinsic fluorescence. [3] It undergoes oxidation only by highly reactive oxygen species (hROS) such as the hydroxyl radical, peroxynitrite, and hROS generated from a peroxidase/H2O2 system. It is inert to hypochlorite ion, nitric oxide, hydrogen peroxide (H2O2), superoxide, and other oxidants. Upon oxidation, HPF is converted to the highly fluorescent molecule fluorescein, with excitation/emission maxima of 490/515 nm, respectively, allowing the simple direct detection of highly reactive biological radicals.

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文献和实验in resolution necessitates preservation of cellular structures close to the native states with minimum artifacts. High-pressure freezing (HPF) that immobilizes molecules in the cell instantaneously has been used to avoid damages caused by convention chemical
What is high-pressure freezing (HPF)? HPF is a method of specimen preparation for electron microscopy (EM) that freezes noncryoprotected samples up to 0.5 mm in thickness without significant ice crystal damage. Other freezing methods
High-Pressure Freezing and Freeze Substitution of Arabidopsis for Electron Microscopy
have not been adequately preserved in a “living state”; hence specimen preparation is of the utmost importance for the success of any electron micrographic study. High-pressure freezing (HPF)/freeze substitution (FS) has long been recognized as the primer technique
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