- ￥3261
- TargetMol
- 美国
- T37821
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
零下80~20°C
- 保质期：
Powder: -20°C for 3 years In solvent: -80°C for 2 years
- 英文名：
AP 14145 hydrochloride
- 库存：
10
- 供应商：
TargetMol
- 规格：
5 mg
Product Introduction
Bioactivity
英文名:AP 14145 hydrochloride
描述:KCa2 (small conductance Ca2+-activated potassium) channel negative allosteric modulator (IC50 = 1.1 μM). Increases the EC50 of Ca2+ on KCa2.3 channels by ~3-fold. Prolongs atrial effective refractory period (AERP) in rats. Reduces atrial fibrillation (AF) duration and prolongs atrial refractoriness without affecting ventricular refractory period in an animal AF model.
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文献和实验Expression Library Screening (Procaryotic) Using AP-Fusion Proteins
production. Following an 8 hour incubation, the filters are marked, removed, washed, blocked and incubated with the AP-fusion protein. Then, the filters are washed again and AP substrate (NBT/BCIP) is added indicating the positive plaques. These positive
a denaturation/renaturation step to the procedure or to perform the protein:protein interaction in-gel without transfer (See In-Gel far-Western Detection below). Denaturation/renaturation is typically accomplished using guanidinium hydrochloride. Blocking Buffer
to the procedure or to perform the protein:protein interaction in-gel without transfer (See In-Gel far-Western Detection below). Denaturation/renaturation is typically accomplished using guanidinium hydrochloride. Blocking Buffer After transferring proteins
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