ML417，化合物ML417，1386162-69-1

Immunofluorescence on ultrathin resin sections

for 10 min. Remove blocking buffer, add 25 µl of the primary antibody solution per coverslip (with a final concentration in the range of 1-5 µg specific IgG/ml) and incubate for 30 -60 min. Wash 5 times with buffer

Immunofluorescence on ultrathin resin sections

blocking buffer, add 25 µl of the primary antibody solution per coverslip (with a final concentration in the range of 1-5 µg specific IgG/ml) and incubate for 30 -60 min. Wash 5 times with buffer and incubate with fluorochrome-labelled

PCR Genotyping of Embryos and Blastocysts

These protocols have come from the transgenic mouse list and are intended as a guide, none of them have been tried in this lab Cell(s) transferred in small volume of water to a .5 ml tube and lysed by rapid freeze / thaw. Buffer was then added