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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
咨询
- 保质期:
咨询
- 英文名:
Glyoxalase I inhibitor;Glyoxalase I inhibitor
- 库存:
咨询
- 供应商:
无锡云萃生物
- CAS号:
221174-33-0
- 规格:
100mg
云萃
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文献和实验antibody dilution buffer: TBS-T supplemented with2% (w/v) BSA. 6. Antibodies: Phospho-b-catenin Ser33/Ser37/Thr41, phospho-glycogen synthase Ser641, and glycogen synthase (CellSignaling Technologies, Beverly, MA); b-catenin and GSK-3b(BD PharMingen, San
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
antibiotic (see Step 51). Ligation buffer (10X) Dilute the 10X stock to 1.15X for Step 18. Magnetic beads, Protein A-conjugated Magnetic beads, Protein G-conjugated PCR buffer (10X) PCR primers, T3 and T7 Phenol
, 1.6 µl 4dNTP mix (25 µM), 2 µl T12 MN primer, 2 µl cDNA, 0.2 µl Taq DNA polymerase (5 U/µl), 1 µl [a- 33P]dCTP aliquot and add 2 µl arbitrary primer (2 pmol/µl) run PCR: 40 cycles (94 °C, 30 sec.) (40 °C, 2 min.) (72 °C, 30 sec.), 1 cycle
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