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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存:
1
- 英文名:
DirectPCR Lysis Reagent (Mouse Tail)
- 保质期:
1年
- 供应商:
北京博蕾德生物科技有限公司
- 保存条件:
2-8℃
- 规格:
100ml
鼠尾直接PCR裂解液
【单瓶试剂,组织裂解+PCR一步到位】
产品关键词:
Viagen Biotech; DirectPCR® 直接PCR;PCR genotyping 基因分型;Proteinase K蛋白酶K;102-T (101-T);
产品介绍:
Viagen DirectPCR® DNA Extraction System(DirectPCR® DNA提取系统)是一种单管系统,用于从小鼠尾巴,耳片,卵黄囊和培养细胞内快递制备DNA。Viagen Biotech, Inc科学家开发的组分(正在申请)允许用DirectPCR® DNA Extraction System获得的DNA提取物与基因分型用的基因组PCR兼容。生物样本的粗提物与许多分子生物学级的试剂比如PCR都难以兼容,部分原因在于粗提物内含有抑制剂。DirectPCR®不仅介导生物样品的快速裂解,而且含有抑制剂,能够有效抑制PCR扩增用粗提裂解物中存在的抑制效应,同时zui大程度保持释放出来的基因组DNA的完整性。申请中的DirectPCR®操作起来,完全消除任何溶液转移或开管步骤,根本上节省了大量时间和精力。
产品特点:
1)省时:更少的动手时间;尾巴裂解液粗提物即可用于PCR;
2)省钱:仅需成本的反应流程;
3)安全:无有机试剂;
4)环保:更少消耗(有机试剂,管子,枪头等等)
5)可靠有效:100%成功率保证高产量
应用实例
简单流程:
1)用DirectPCR®裂解鼠尾;
2)85℃孵育45min;
3)取1μl裂解液进行PCR基因型研究
订购信息:
| 品牌 | 产品名称 | 货号 | 规格 |
| Viagen Biotech | DirectPCR Lysis Reagent (Mouse Tail) | 101-T | 50ml(up to 250 tails) |
| Viagen Biotech | DirectPCR Lysis Reagent (Mouse Tail) | 102-T | 100ml(up to 500 tails) |
| Viagen Biotech | Genomic PCR Grade Proteinase K Solution | 501-PK | 5ml |
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文献和实验,and the cell pellets were frozen and stored at –80ºC. Lysis Lysis was accomplished with B-PER reagent supplemented with 1/50 vol 10-mg/ml chicken egg-white lysozyme (stored at –80ºC in aliquots),1/125 vol 5-mg/ml DNaseI (in 20 mM sodium acetate,pH adjusted
Isolation of RNA from Difficult Tissues
-binding methods, as well as with methods that use oligo d(T) selection of mRNA. Tissue is simply removed from RNAlater and processed like fresh tissue in the RNA isolation lysis solution. There is no need to freeze and grind the tissue using a mortar
DNA Fragmentation Assays for Apoptosis Protocol
before colorimetric assessment.Stock IV: Lysis buffer at pH 8.0 5mM Tris-HCl 20mM EDTA 0.5% Triton X-100Stock V: Buffer at pH 8.0 10mM Tris-HCl 1mM EDTAStock VI: Diphenylamine reagent (light sensitive) 1.5g of diphenylamine (steam-distilled) 100ml acetic acid











