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- 供应商:
赛默飞世尔科技
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有
- 英文名:
L GLUTAMINE, 100X 100ML
Gibco™ L-glutamine 200 mM Solution is a stock solution ready-to-use as a cell culture supplement. The optimal use concentration is dependent upon the cell type and medium used to culture the cells, but generally falls in the range of 2 – 6 mM.
Product Use
For in vitro diagnostic use.
Dual-site cGMP Manufacturing and Quality System
Gibco™ MEM L-glutamine is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers an identical Gibco™ L-glutamine product made in our Scotland facility (25030-123). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
在中国境内, 本产品仅限于非临床科研用途, 产品信息以中文为准。 (In China territory, the product is for Research Use only in non-clinical unit, product information in Chinese shall prevail.)
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文献和实验Protocol for competitive RT-PCR
that containes a T7 RNA promoter sequence. The in vitro transcription of the cloned fragments is performed using T7 RNA polymerase (e.g. Gibco BRL). The internal standard RNA is then treated with RNase-free DNase I (e.g. Gibco BRL) to remove the plasmid DNA
2.mESC株 RB1 12保持于ES细胞培养基包括Knock-out™培养液(10829-018;Gibco,UK),辅以15%ES标准的FCS(10439-024;Gibco),2mM L-谷氨酰胺(15039-027;Gibco),0.1 mM 2 -巯基乙醇(M7522;Sigma),1%氨基酸液(11140-035;Gibco),100 U / mL青霉素和100μg/ mL链霉素(15070-063;Gibco)。 提示: 小鼠胚胎成纤维细胞(mEF
供应商帮你找。至于要不要加其他成分,请根据实验目的,请教身边的老师。一般每个科室细胞培养 实验室都有操作规范。 一般培养基配制每升加入2.0-2.2g碳酸氢钠,2.7-5.4gHepes。其他要补充的要看你所养细胞要求,例如添加L-谷氨酰胺。因为Glutamine在液体中很易分解,且glutamine是细胞合成核酸和蛋白质必需的氨基酸,在缺少谷氨酰胺时,细胞会生长不良而死亡。建议你仍补加终浓度为2mM的glutamine。配制培养基用1000的水配。
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