Tissue: oesophagus; Tumor: carcinoma, squamous cell. Karotype: aneuploid. A p53 mutation at the splice acceptor site of intron 6 and a 12 fold amplification of c-erb B have been reported. The cells express a large amount of epidermal growth factor receptors. Passage number: 340. 组织:食道;肿瘤:癌,鳞状细胞。核型:非整倍体。已经报道了内含子 6 剪接受体位点的 p53 突变和 c-erb B 的 12 倍扩增。细胞表达大量表皮生长因子受体。段落编号:340。
Growth Properties
Polygonal
Recommended Medium And Supplement
RPMI 1640 + Ham’s F12 (1: 1) + 2% FBS + 2 mM L-Glutamine; Split confluent cultures 1: 5-1: 10 using trypsin/EDTA; seed at 1×104 cells/cm2; 37℃, 5% CO2.