MONO-MAC-6 is a human monocyte cell line derived from patients with acute monocytic leukemia. MONO-MAC-6 cells possess phenotypic and functional features of mature monocytes such as CD14 expression, phagocytic capacity, and cytokine production. Acute monocytic leukemia (AMoL or AML-M5) is a type of acute myeloid leukemia characterized by peripheral monocytosis. More than 80% of the nucleated cells of AMoL are of monocytic origin. Studies demonstrate that MONO-MAC-6 cells exhibit similar monocyte-endothelial interaction to human monocytes.
Why choose MONO-MAC-6 from AcceGen?
MONO-MAC-6 cells are established from the peripheral blood of a 64-year-old man with relapsed acute monocytic leukemia. Cells are cryopreserved to ensure the best viability. Each vial contains more than 1×10^6 cells in 1ml volume. MONO-MAC-6 cells carry a t(9;11)(p22;q23) translocation, which results in the fusion of KMT2A-MLLT3 gene (MLL-MLLT3; MLL-AF9). Subculture cells at a split ratio of 1:3 to 1:5 with a cell seeding density between 0.3×10^6 to 1×10^6 cells/ml.