GFP-bAECs are selected from bAECs transfected using lentivirus expressing GFP using Puromycin. The cells are shipped in a frozen vial(the cells are provided @ passage 2). Endothelial Growth Medium is recommended for the expansion of GFP-bAECs and these cells can be propagated to at least 12 population doubling times without losing their morphologic and phenotypic characteristics when cultured following the detailed protocol described below. GFP-bAECs 选自使用嘌呤霉素表达 GFP 的慢病毒转染的 bAECs。细胞装在冷冻小瓶中运输(细胞在第 2 代提供)。建议将内皮生长培养基用于 GFP-bAECs 的扩增,并且这些细胞在按照下面描述的详细方案进行培养时,可以传播到至少 12 倍的群体倍增倍,而不会失去其形态学和表型特征。
Quality Control
All cells test negative for mycoplasma, bacteria, yeast, and fungi.