Mouse Liver Microvascular Endothelial cells (mLivMECs) are isolated from livers of 3-week old male C57BL/6 mice and GFP expressing mLivMECs-GFP were selected from puromycin resistant cells after transfected with lentiviral expressing GFP. Cells are shipped in a frozen vial (provided @ passage 3, with > 5 x 105cells/vial). mLivMECs-GFP are cultured with specially formulated Endothelial Growth Medium. Since the cells have limited proliferation capacity (cells may be expanded for a maximum of 2-3 passages) in vitro, long term culture of mLivMECs is not recommended, even cultured following the detailed protocol described below). 小鼠肝脏微血管内皮细胞 (mLivMECs) 是从 3 周龄雄性 C57BL/6 小鼠的肝脏中分离出来的,表达 GFP 的 mLivMECs-GFP 是从用表达 GFP 的慢病毒转染后的嘌呤霉素抗性细胞中选出的。细胞装在冷冻小瓶中(第 3 代提供,> 5 x 105 个细胞/小瓶)。 mLivMECs-GFP 与特殊配方的内皮生长培养基一起培养。由于细胞在体外的增殖能力有限(细胞最多可扩增 2-3 代),因此不建议长期培养 mLivMEC,即使按照下面描述的详细方案进行培养。
Quality Control
All cells test negative for mycoplasma, bacteria, yeast, and fungi.