PoASMCs-RFP cells are selected from RFP-lentiviral transfected PoASMCs resistant to PUROMYCIN. The cells are shipped in a frozen vial(the cells are provided @ passage 3). Smooth Muscle Cells(SMCs) Growth Medium is recommended for the expansion of PoASMCs-RFP and these cells can be propagated to extra 3-4 passages without losing their morphologic and phenotypic characteristics when cultured following the detailed protocol described below. PoASMCs- rfp细胞是从rfp慢病毒转染的对嘌呤霉素耐药的PoASMCs细胞中筛选的。这些细胞装在冰冻的小瓶中运输(细胞在第3代时提供)。平滑肌细胞(SMCs)生长培养基可用于PoASMCs-RFP的扩增,当按照下面描述的详细方案培养时,这些细胞可扩增3-4代,而不会失去其形态和表型特征。
Quality Control
All cells test negative for mycoplasma, bacteria, yeast, and fungi.