BKS DB MOUSE PANCREATIC MICROVASCULAR ENDOTHELIAL CELLS
CAS号:
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保质期:
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供应商:
北京百奥创新科技有限公司
保存条件:
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Product Name
BKS DB Mouse Pancreatic Microvascular Endothelial Cells
Cat.No
ABC-TC5489
Species
BKS DB Mouse
Size/Quantity
1 vial
Shipping Info
Dry Ice
Storage
Liquid Nitrogen
Description
BKS db Mouse Pancreatic Microvascular Endothelial Cells are isolated from the pancreatic microvascular of Mice homozygous for the diabetes spontaneous mutation (Lepr/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoRIemic. BKS db Mouse Pancreatic Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in AcceGen’s Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×106 cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody. BKS db 小鼠胰腺微血管内皮细胞是从糖尿病自发突变 (Lepr/db) 纯合子小鼠的胰腺微血管中分离出来的,表现为病态肥胖、慢性高血糖症、胰腺β细胞萎缩并变得低血。 BKS db 小鼠胰腺微血管内皮细胞在预涂有明胶溶液的 T25 组织培养瓶中生长 2 分钟,然后在 AcceGen 的完全培养培养基中培养 3-7 天。然后扩大文化。运输前,将第 3 代细胞从烧瓶中取出并立即冷冻保存在小瓶中。每个小瓶每毫升至少含有 0.5×106 个细胞。我们用于分离原代内皮细胞的方法是基于已建立的方法和我们专有的方法相结合而开发的。这些细胞预涂有 PECAM-1 抗体,然后应用预涂有二抗的磁珠。