Rabbit Primary Cardiac Fibroblasts from AcceGen are isolated from heart tissue of New Zealand White Rabbit. Rabbit Primary Cardiac Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5×106 cells per ml and is delivered frozen. 本研究从新西兰大白兔心脏组织中分离得到兔原代心脏成纤维细胞。兔原代心脏成纤维细胞在T75组织培养瓶中培养2分钟,用明胶包衣液包衣,在完全培养基中培养3-7天。文化被扩展。在运输之前,细胞从烧瓶中分离出来,并立即冷冻保存在小瓶中。每个小瓶至少包含0.5×106细胞每毫升和交付冷冻。