Rat Dorsal Root Ganglion Neurons are suspensions of high quality sensory neurons prepared by standardized methods, and are ready for immediate culture. Each vial of dorsal root ganglia cells contains approximately 200,000 cells in 0.25 ml suspension. Cell death will occur during the first few days after plating and debris will be observed. This is normal. After approximately 4 days in culture, the cells will form a neurite network and by the 7th day, debris will be minimal. In the absence of mitotic inhibitors, the neurons tend to cluster (ganglionate) and detach from the substrate. A ganglion is a group of nerve cells forming a nerve center, especially one located outside the brain or spinal cord. Dorsal root ganglion, also called spinal ganglion, is the ganglion of the posterior root of each spinal segmental nerve, containing the cell bodies of the unipolar primary sensory neurons. Dorsal root ganglion cells are pseudounipolar cells. Pseudounipolar cells have 2 axons rather than an axon and dendrite. One axon extends centrally toward the spinal cord; the other axon extends toward the skin or muscle. 大鼠背根神经节神经元是用标准方法制备的高质量感觉神经元悬浮液,可直接培养。 每瓶背根神经节细胞含有约20万个细胞,0.25 ml悬液。在电镀后的头几天内会发生细胞死亡,并观察到碎片。这是正常的。培养大约4天后,细胞会形成一个神经突网络,到第7天,碎片将会最小。 在缺乏有丝分裂抑制剂的情况下,神经元倾向于聚集(神经节)并与底物分离。神经节是形成神经中枢的一组神经细胞,尤指位于大脑或脊髓外的神经细胞。 背根神经节又称脊髓神经节,是每个脊髓节段神经后根的神经节,包含单极初级感觉神经元的细胞体。背根神经节细胞为伪单极细胞。假单极细胞有两个轴突,而不是一个轴突和树突。一个轴突向中央延伸至脊髓;另一个轴突延伸到皮肤或肌肉。