HUVECs were infected with Lentiviral particles expressing human VEGFR2 specific shRNAs and GFP @ passage 1. Puromycin resistant HUVECs-shRNAVR2-GFP cells were selected and shipped in frozen vials (cells are provided @ passage 3). Endothelial Growth Medium is recommended for cell culture. 用表达人VEGFR2特异shRNAs和GFP的慢病毒颗粒感染HUVECs。选择耐Puromycin的HUVECs-shRNAVR2-GFP细胞,装在冷冻小瓶中(细胞在第3代时提供)。细胞培养推荐使用内皮生长介质。
Quality Control
All cells test negative for mycoplasma, bacteria, yeast, and fungi.