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- 详细信息
- 文献和实验
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咨询
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- 英文名:
SIGMA
- 库存:
999
- 供应商:
麦飞生物
- CAS号:
无
- 规格:
C9995-200UL
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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
The production of monoclonal antibody by hybridoma
inactivated and pre-filtered commercial Fetal Bovine serum (FBS) or Horse Serum (HS) stored in the refrigerator at 40C. Must be pretested for myeloma growth from single cells. L-glutamine, 200mM, 100X solution stored at -200C
Protocol for intracytoplasmic staining of cytokines for FACS analysis
+ 1% BSA, + 5% heat inactivated normal rabbit serum (HINRS). 9) Label with antibodies to surface molecules that are resistant to fixation (30 mins 4 deg C) eg. anti-CD4 (H129.19-Quantum Red; Sigma R 3637) for FL3 anti-CD44 (Pgp-1:IM
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