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VTi 90, VTi 65, VTi 65.1, VTi 65.2, NVT 100, NVT 90, NVT 65.2, VTi 80, TLN-120, TLN-100, TLN-80 - 联系方式:
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文献和实验Joel Huberman DNA isolation procedure with modifications by Bonny Brewer
volume of 4.0 ml (or 4.08 g) plus 4.2 g of CsCl. This volume will fill one VTi65 heat seal tube.) Encourage the CsCl to dissolve by gentle mixing. Don't mix vigorously or foaming will result. 12. After the CsCl has dissolved, measure the volume and add
RUVKUN Antibody Staining Protocol revised 10/29/90
ul glycerol (65 C) -add 30 ul 100 mM Tris Cl pH 9.5 -add 0.2 ul 1 mg/ml DAPI or Hoechst stain (wear gloves, CARCINOGENS) Add an equal volume of stained worms and mix with pipette tip. -use nail polish to seal slides
proteinase K buffer (0.2 M Tris (pH 8.0), 0.1 M EDTA, 1% Sarkosyl, 100 g/ml proteinase K) Pour into 10 ml test tube. Incubate at 45-50 C for 1 hr. Spin 10 min at top speed in table top centrifuge (~3000 rpm) Decant supernatant to a fresh
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