可靠地纯化多种样本中的 RNA TRIzol LS 试剂可处理多种液体样本(样本量多达 0.25 mL)。TRIzol LS 试剂的浓度不同于标准 TRIzol 试剂,可处理较大的样本。与标准 TRIzol 试剂一样,TRIzol LS 试剂可通过在样本均质化期间高效抑制核糖核酸酶活性而维持所得 RNA 制剂的完整性。TRIzol LS 试剂用法简单,因此可同时处理大量样本。整个过程不到 1 小时。通过 TRIzol LS 试剂分离的总 RNA 不含蛋白和 DNA 污染。
其配方可进行多次分离 TRIzol LS 试剂使您可连续沉淀单份样本中的 RNA、DNA 和蛋白。使用 TRIzol LS 试剂均质化样本后,加入氯仿,使匀浆分离成透明的上层水层(含 RNA)、界面和红色下层有机层(含 DNA 和蛋白)。使用异丙醇从水层中沉淀出 RNA。使用乙醇从水层/有机层界面中沉淀出 DNA。通过异丙醇沉淀从苯酚-乙醇层中沉淀出蛋白。洗涤沉淀出的 RNA、DNA 或蛋白以去除杂质,然后重悬以用于下游应用。
TRIzol LS reagent is a complete ready-to-use reagent optimized for separating high-quality total RNA from multiple liquid samples or simultaneously separating RNA, DNA, and proteins. The single-phase solution of phenol and guanidine isothiocyanate can usually separate individual components of RNA, DNA, and protein from liquid samples of human, animal, plant, yeast, bacterial, and viral sources within one hour. The main characteristics of TRIzol LS reagent include:
Its formula is suitable for liquid samples, such as serum or viral preparations
• Facilitate the recovery of RNA, DNA, and proteins from single liquid samples
Even difficult to treat biological liquids still have excellent cracking ability