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- 详细信息
- 技术资料
- 形态:
液体
- 保存条件:
冷冻(-20℃)
- 克隆性:
多克隆
- 适应物种:
兔
- 保质期:
一年
- 抗原来源:
POLR2A
- 库存:
200
- 供应商:
上海酶联生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
仅供科研使用
- 抗体英文名:
Anti-POLR2Arabbitpolyclonalantibody
- 抗体名:
兔抗POLR2A多克隆抗体
- 规格:
100ul/200ul/25ul
| 规格: | 100ul | 产品价格: | ¥1560.0 |
|---|---|---|---|
| 规格: | 200ul | 产品价格: | ¥2500.0 |
| 规格: | 25ul | 产品价格: | ¥500.0 |
中文名称: 兔抗POLR2A多克隆抗体
英文名称:Anti-POLR2Arabbitpolyclonalantibody
别名:RPB1;RPO2;POLR2;POLRA;RPBh1;RPOL2;RpIILS;hsRPB1;hRPB220
相关类别:一抗
储存:冷冻(-20℃)避光
宿主:Rabbit
抗原:POLR2A
反应种属:Human,Mouse,Rat
标记物:Unconjugate
克隆类型:rabbitpolyclonal
技术规格
英文名称:Anti-POLR2Arabbitpolyclonalantibody
别名:RPB1;RPO2;POLR2;POLRA;RPBh1;RPOL2;RpIILS;hsRPB1;hRPB220
相关类别:一抗
储存:冷冻(-20℃)避光
宿主:Rabbit
抗原:POLR2A
反应种属:Human,Mouse,Rat
标记物:Unconjugate
克隆类型:rabbitpolyclonal
技术规格
| Background: | RNApolymeraseII(RNAPII)isalargemulti-pro teincomplexthatfunctionsasaDNA-dependen tRNApolymerase,catalyzingthetranscriptionof DNAintoRNAusingthefourribonucleosidetripho sphatesassubstrates.Thelargestsubunit,RNAPII subunitB1(Rpb1),alsoknownasRNAPIIsubunitA (POLR2A),containsauniqueheptapeptidesequence (Tyr1,Ser2,Pro3,Thr4,Ser5,Pro6,Ser7),whichisrepeate dupto52timesinthecarboxy-terminaldomain(CTD) oftheprotein.ThisCTDheptapeptiderepeatissubjec ttomultiplepost-translationalmodifications,which dictatethefunctionalstateofthepolymerasecomplex. PhosphorylationoftheCTDduringtheactivetranscript ioncycleintegratestranscriptionwithchromatinremo delingandnascentRNAprocessingbyregulatingthere cruitmentofchromatinmodifyingenzymesandRNApro cessingproteinstothetranscribedgene.Duringtranscri ptioninitiation,RNAPIIcontainsahypophosphorylated CTDandisrecruitedtogenepromotersthroughinteracti onswithDNA-boundtranscriptionfactorsandtheMedia torcomplex.TheescapeofRNAPIIfromgenepromoters requiresphosphorylationatSer5byCDK7,thecatalyticsu bunitoftrans criptionfactorIIH(TFIIH).PhosphorylationatSer5mediat estherecruitmentofRNAcappingenzymes,inadditionto histoneH3Lys4methyltransferases,whichfunctiontoreg ulatetranscriptioninitiationandchromatinstructure.Aft erpromoterescape,RNAPIIproceedsdownthegenetoan intrinsicpausesite,whereitishaltedbythenegativeelonga tionfactorsNELFandDSIF.Atthispoint,RNAPIIisunstablea ndfrequentlyabortstranscriptionanddissociatesfromth egene.Productivetranscriptionelongationrequiresphos phorylationatSer2byCDK9,thecatalyticsubunitoftheposi tivetranscriptionelongationfactorP-TEFb.Phosphorylation atSer2createsastabletranscriptionelongationcomplexan dfacilitatesrecruitmentofRNAsplicingandpolyadenylatio nfactors,inadditiontohistoneH3Lys36methyltransferases, whichfunctiontopromoteelongation-compatiblechromatin. |
| Applications: | WB |
| Nameofantibody: | POLR2A |
| Immunogen: | FusionproteinofhumanPOLR2A |
| Fullname: | polymerase(RNA)II(DNAdirected)polypeptideA,220kDa |
| Synonyms: | RPB1;RPO2;POLR2;POLRA;RPBh1;RPOL2;RpIILS;hsRPB1;hRPB220 |
| SwissProt: | P24928 |
| WBPredictedbandsize: | 270kDa |
| WBPositivecontrol: | HeLacells |
| WBRecommendeddilution: | 500-2000 |
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技术资料兔抗POLR2A多克隆抗体
¥500 - 2500









