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大小: 100ul 甘油中的杂交瘤培养基。
宿主:小鼠(IgG2a)
已知特异性:人类。
小鼠和大鼠 - 未经测试。
推荐稀释:
蛋白质印迹 1:1,000
免疫组化待定。
这些工作条件仅作为建议提供。每个用户都应该为自己的实验确定最佳条件。
资料来源:该小鼠单克隆抗体使用肽(氨基酸 23-39)通过添加 C 末端半胱氨酸与 KLH 偶联而产生至细胞外结构域。杂交瘤培养基从在培养基中生长的细胞获得并用硫酸铵浓缩。
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文献和实验TRAF‐Mediated TNFR‐Family Signaling
Figure 11.9.1 Domain organization of mammalian tumor‐necrosis factor receptor (TNFR)‐associated factors (TRAFs). The TRAF family consists of seven proteins. Each TRAF, except TRAF1, has a RING‐finger domain
Production and Characterization of Receptor-Specific TNF Muteins
Tumor necrosis factor (TNF) is a pleiotropic cytokine with a wide range of biological activities including cytotoxicity, immune-cell proliferation, and mediation of inflammatory responses. Mutational analysis of mature TNF has been facilitated
Detection of TNF and TNF Receptor mRNA in Cells and Tissues
We describe a semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) technique for the quantification of the messenger RNA of human and murine tumor necrosis factor α (TNFα) and related receptors. This protocol can be adapted
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