GAPDH Monoclona

Product name	GAPDH Monoclonal Antibody
Isotype	IgG
Form	Liquid
Tested applications	WB
Species reactivity	Mouse,Rat,Human,Fish (predicted_ Horse,Chicken,Guinea pig,Hamster,Cat,Dog,Pig,Xenopus laevis,Monkey,Zebrafish,Baboon,Xenopus tropicalis)
Host Species	Mouse
Purity	Affinity purification

Immunogen	Recombinant protein of human GAPDH
Calculated molecular weight	36kDa
Observed molecular weight	36kDa
Swiss Prot	P04406
Gene ID (NCBI)	2597
Alternative Names	G3PD,GAPD,HEL-S-162eP,GAPDH

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

WB	1:2000-1:10000

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.

Storage instructions

Store at -20℃. Avoid freeze / thaw cycles.PBS with 1% BSA, 0.03% Proclin300 and 50% Glycerol.