Lipolysis Assay Kit (Fluorometric) ab185434 is simple, easy-to-use and highly sensitive.
This lipolysis assay measures glycerol released from 3T3-L1 cells as early as 1 hour after induction of lipolysis. It is suitable for measuring trace amounts of glycerol from samples. In the assay, glycerol reacts with Glycerol Enzyme Mix to form an intermediate that in turn reacts with Glycerol Developer and PicoProbe™ to generate the fluorescence product. The fluorescence intensity is directly proportional to the amount of glycerol.
The lipolysis assay kit can detect less than 20 pmol of glycerol.
说明
This product is manufactured by BioVision, an Abcam company and was previously called K578 PicoProbe™ Lipolysis (3T3-L1) Fluorometric Assay Kit. K578-100 is the same size as the 100 test size of ab185434.
Lipolysis is the hydrolysis of triglycerides within the cell into glycerol and free fatty acids. The glycerol and free fatty acids are then released into the bloodstream or culture media. Lipolysis occurs in essentially all cells, but is most abundant in white and brown adipose tissue.