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- 详细信息
- 文献和实验
- 技术资料
- 靶点:
TDG
- 应用范围:
WB 1:2000, IHC 1:150
- 宿主:
Mouse
- 适应物种:
Human, Mouse, Rat
- 保质期:
1-2年
- 供应商:
安诺伦(北京)生物科技有限公司
- 标记物:
Unconjugated
- 克隆性:
Monoclonal Antibody
- 保存条件:
Stable for 1 year at -20°C from date of shipment. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
- 形态:
Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
- 亚型:
IgG1
- 免疫原:
Human recombinant protein fragment corresponding to amino acids 160-253 of human TDG(NP_003202) produced in E.coli.
- 规格:
100 ug
产品概述:Carrier-free (BSA/glycerol-free) TDG mouse monoclonal antibody, clone LBI3C11
总结:The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12. [provided by RefSeq, Jul 2008]
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文献和实验Cloning of Variable Domains from Mouse Hybridoma by PCR
of thoroughly validated oligonucleotide primers for a PCR procedure to isolate and clone the DNA of antibody Fv fragments (antigen binding region) from mouse and rat hybridoma cell lines. The protocol includes a colony staining assay to identify E. coli clones
diseases as well as for cancer during aging. We established a quantitative method for in situ detection of 8-oxoG in mitochondrial DNA in a single-cell level using a monoclonal antibody. Specific detection of 8-oxoG in mitochondrial DNA was confirmed by pre
T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
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