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- 详细信息
- 文献和实验
- 技术资料
- 靶点:
KRT16
- 应用范围:
WB 1:2000, IHC 1:150
- 宿主:
Mouse
- 适应物种:
Human, Rat
- 保质期:
1-2年
- 供应商:
安诺伦(北京)生物科技有限公司
- 标记物:
Unconjugated
- 克隆性:
Monoclonal Antibody
- 保存条件:
Stable for 1 year at -20°C from date of shipment. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
- 形态:
Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
- 亚型:
IgG1
- 免疫原:
Full length human recombinant protein of human KRT16 (NP_005548) produced in E.coli.
- 规格:
100 ug
产品概述:Carrier-free (BSA/glycerol-free) KRT16 mouse monoclonal antibody, clone LBI2E6
总结:The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region of chromosome 17q12-q21. This keratin has been coexpressed with keratin 14 in a number of epithelial tissues, including esophagus, tongue, and hair follicles. Mutations in this gene are associated with type 1 pachyonychia congenita, non-epidermolytic palmoplantar keratoderma and unilateral palmoplantar verrucous nevus. [provided by RefSeq, Jul 2008]
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文献和实验The production of monoclonal antibody by hybridoma
The production of monoclonal antibody by hybridoma fusion: Immortalization of sensitized B lymphocytes from immune mice. Overview
secondary antibody review -- data from 99 publications
Signaling 18 IgG Alexa Fluor 488 immunocytochemistry 1:2000 18 IgG Alexa Fluor 568 immunocytochemistry 1:2000 19 donkey IgG HRP western blot 1:3000 detect antibody binding in mouse epithelial cells Amersham Biosciences 19 goat
5 and 6)- Monoclonal antibody clone D6/16-B4.- Source: Roche Diagnostics- Dilution 1:25.- Antigen retrieval method: HIER (pressure cooking) using EDTA.- Staining pattern: Cytoplasmic, with a fibrillary quality, often with subplasmalemmal or perinuclear accentuation
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