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- Lucigen
- 进口试剂
- 60435-1
- 2025年07月12日
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- 文献和实验
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- 保存条件：
-80º C
- 库存：
100
- 供应商：
远慕生物
- 规格：
12 rxns (SOLOs)
美国Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品，凭借其独到的产品技术，过硬的产品质量，良好的产品服务赢得了全球广大用户的信赖。
Lucigen Corporation is working to improve people’s lives by providing quality products and services to life science and healthcare professionals. Founded in Wisconsin in 1998, Lucigen offers products and services that enable life-science professionals to perform their research and testing more efficiently and effectively. The company has grown to manufacture and sell over 325 biomedical research products and services to customers worldwide, all under an ISO 13485 compliant quality system. Core competencies include enzyme evolution, protein expression, cloning, competent cells, next gen sequencing, and molecular diagnostics. "
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文献和实验TOP10 chemically competent cells
Overview This protocol is a variant of the Hanahan protocol [1] using CCMB80 buffer for DH10B, TOP10 and MachI strains. It builds on Example 2 of the Bloom05 patent as well. This protocol has been tested on TOP10, MachI and BL21(DE3) cells
Transforming chemically competent cells
Method Thaw TSS cells on ice. Add DNA, pipette gently to mix (1μl of prepped plasmid is more than enough). Note: If you are adding small volumes (~1μl), be careful to mix the culture well. Diluting the plasmid
Preparing chemically competent cells
Materials Plate of cells to be made competent TSS buffer LB media Ice Glassware & Equipment Falcon tubes 500μl Eppendorf tubes, on ice 200ml conical flask 200μl pipetman or repeating
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