5 - FITC Microscale Protein Labeling Kit *Ultra Convenient*
CAS号:
5 - FITC Microscale Protein Labeling Kit *Ultra Convenient*
保质期:
详见说明
供应商:
齐源生物
保存条件:
-20°C
规格:
1 kit
5 - FITC 微量蛋白质标记试剂盒、5 - FITC Microscale Protein Labeling Kit *Ultra Convenient*详细如下: 5-FITC 比 6-FITC 异构体更广泛地用于蛋白质标记。FITC-蛋白偶联物适用于免疫荧光染色、原位杂交、流式细胞术和其他生物学应用。该试剂盒具有进行偶联反应和纯化 FITC 蛋白偶联物的所有必要组件。该试剂盒适用于每个反应的三个偶联反应,最多可标记 200 µg IgG。 异硫qing酸荧光素 (FITC) 仍然是最流行的荧光标记试剂之一,这可能是由于该材料的低成本。5-FITC 和 6-FITC 具有非常相似的吸收和荧光光谱。然而,异构体可能在它们与蛋白质的结合和反应性方面有所不同,并且缀合物可能在不同的色谱条件下洗脱或在电泳凝胶中以不同的方式迁移。 注意:AnaTag 试剂盒不适用于肽标记。 Resuspend the gel in the spin column (component C) by inverting sharply several times. Avoid bubbles. Remove the top cap of the column, and then cut its bottom tip. Place the column into a wash tube (component F) and centrifuge at 1,000 x g for 2 min. Discard the eluted buffer. Exchange the gel-packing buffer by adding 500 uL of elution buffer (component E) to the spin column and centrifuge at 1,000 x g for 1 min. Discard the eluent. Repeat the above step three times. Place the spin column into a clean collection tube (component G). Apply the reaction mixture from Step 3 to the center of gel bed surface. Centrifuge the column at 1,000 x g for 4 min. The dye-protein conjugate is in the collection tube. The degree of substitution (DOS) of the conjugate should be determined according to the Appendix.