Rh110 组织蛋白酶 L 检测试剂盒 荧光法、Rh110 Cathepsin L Assay Kit *Fluorimetric*详细如下: Rh110 Cathepsin L 活性检测试剂盒提供了一种方便的检测方法,用于筛选 Cathepsin L 抑制剂或使用荧光底物检测酶活性。在被组织蛋白酶 L 切割后,该底物会产生带有亮绿色荧光的 Rh110(罗丹明 110)荧光团,可以在激发/发射 = 490/520 nm 下检测到。Rh110 更长的波长光谱和更高的消光系数提供更高的灵敏度和更少的其他反应组分的干扰。 组织蛋白酶是一类球状溶酶体蛋白酶,在哺乳动物细胞更新中起着至关重要的作用。它们降解多肽并通过它们的底物特异性来区分。组织蛋白酶 L 是一种溶酶体内肽酶,是半胱氨酸蛋白酶类木瓜蛋白酶家族的成员。 Add test compounds and diluted enzyme solution to the microplate wells. The suggested volume of enzyme solution for a 96-well plate is 40 uL/well and test compound is 10 uL/well. Simultaneously set up the following control wells, as deemed necessary: Positive control contains the enzyme without test compound. Inhibitor control contains Cathepsin L enzyme and inhibitor. Vehicle control contains Cathepsin L enzyme and vehicle used in delivering test compound (e.g. DMSO, concentration not to exceed 1%). Test compound control contains assay buffer and test compound. Some test compounds have strong autofluorescence and may give false results. Substrate control contains assay buffer. Using the assay buffer, bring the total volume of all controls to 50 uL. Optional: Pre-incubate the plate for 10 min. at assay temperature. Any temperature (the assay temperature) from room temperature to 37 ℃ may be used, as long as the subsequent incubations are performed at the same temperature.