Rh110 肠激酶检测试剂盒 荧光法、Rh110 Enterokinase Activity Assay Kit *Fluorimetric*详细如下: Rh110 肠激酶检测试剂盒采用荧光底物检测肠激酶活性检测。蛋白酶裂解后,该底物会产生具有亮绿色荧光的 Rh110(罗丹明 110)荧光团,可在激发/发射 = 490/520 nm 处检测到。检测限:0.31 ng/ml、Rh110 的更长波长光谱和更高消光系数提供更高的灵敏度和更少的其他反应组分的干扰。检测以方便的 96 孔微孔板形式进行。 肠激酶或肠肽酶是由十二指肠壁中的细胞产生的异二聚丝氨酸蛋白酶。它将胰蛋白酶原转化为胰蛋白酶,间接激活许多胰腺消化酶。肠激酶是肠道消化蛋白质的关键酶。它的缺乏可能导致严重的蛋白质吸收不良,从而导致发育和生长受损。 Note: Warm all kit components until thawed to room temperature before starting the experiments. 1X assay buffer: Add 10 mL of 2X assay buffer (Component D) to 10 mL of deionized water. Enterokinase substrate solution: Dilute enterokinase substrate (Component A) 100-fold in 1X assay buffer. Refer to Table 1. For each experiment, prepare fresh substrate solution. If not using the entire plate, adjust the amount of substrate to be diluted accordingly. Enterokinase diluents: Dilute the enterokinase enzyme (Component C) 100-fold in 1X assay buffer. This amount of enzyme is enough for a full 96-well plate. If not using the entire plate, adjust the amount of enzyme to be diluted accordingly. Note: Prepare enzyme diluents immediately before use. Do not vortex the enzyme solution. Prolonged storage or vigorous agitation of the diluted enzyme will cause denaturation. Store the enzyme solution on ice. Enterokinase inhibitor, E-64: Dilute the 50 mM inhibitor solution (Component E) 10-fold in 1X assay buffer to get 5 mM diluted inhibitor solution. Add 10 ul of the diluted inhibitor into each of the inhibitor control well.