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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
FDG β-Galactosidase Assay Kit *Fluorimetric*
- CAS号：
FDG β-Galactosidase Assay Kit *Fluorimetric*
- 保质期：
详见说明
- 供应商：
齐源生物
- 保存条件：
-20°C
- 规格：
1 kit
FDG ß-半乳糖苷酶检测试剂盒 荧光法、FDG β-Galactosidase Assay Kit *Fluorimetric*详细如下：
FDG β-半乳糖苷酶检测试剂盒提供了一种灵敏的荧光底物，用于检测转染细胞中的 β-半乳糖苷酶活性。在存在 β-半乳糖苷酶的情况下，无色荧光素二-β-D 吡喃半乳糖苷 (FDG) 底物被水解成可在 Ex/Em = 490/520 nm 处检测到的荧光素。检测限：100 pg/ml、由大肠杆菌中的 lacZ 基因编码的 β-半乳糖苷酶被广泛用作研究哺乳动物细胞中基因表达、蛋白质-蛋白质相互作用和转染效率标准化的报告酶。
Prepare assay buffer: Prepare fresh assay buffer for each experiment according to Table 1. Use this DTT-containing assay buffer in all the following steps.
Prepare cell extracts: Aspirate culture medium from cells. Wash cells by using PBS or Hanks’ Balanced Salts Solution (HBSS). Prepare lysis buffer by adding a final concentration of 0.1% Triton X-100 (Component F) to assay buffer. Add 50 uL of prepared lysis buffer to the microplate wells. Incubate at room temperature for 10 min to allow cell lysis.
Note: If the cell extracts are prepared in culture media, we recommend running a medium control with the ß-galactosidase standard to establish the effects of the medium on assay performance.
ß-galactosidase substrate solution: Dilute ß-galactosidase substrate (Component A) 1:200 in assay buffer. For each experiment, prepare new substrate solution.
Prepare dilutions of ß-galactosidase standard (optional): Dilute ß-galactosidase (Component C) to 500 ng/mL (1:200) in assay buffer containing Triton X-100. Then make 3-fold serial dilutions to get concentration of 166.66, 55.55, 18.52, 6.17, 2.06, 0.686 ng/mL, include a blank control.
FDG β-半乳糖苷酶检测试剂盒提供了一种灵敏的荧光底物，用于检测转染细胞中的 β-半乳糖苷酶活性。在存在 β-半乳糖苷酶的情况下，无色荧光素二-β-D 吡喃半乳糖苷 (FDG) 底物被水解成可在 Ex/Em = 490/520 nm 处检测到的荧光素。检测限：100 pg/ml、由大肠杆菌中的 lacZ 基因编码的 β-半乳糖苷酶被广泛用作研究哺乳动物细胞中基因表达、蛋白质-蛋白质相互作用和转染效率标准化的报告酶。
Prepare assay buffer: Prepare fresh assay buffer for each experiment according to Table 1. Use this DTT-containing assay buffer in all the following steps.
Prepare cell extracts: Aspirate culture medium from cells. Wash cells by using PBS or Hanks’ Balanced Salts Solution (HBSS). Prepare lysis buffer by adding a final concentration of 0.1% Triton X-100 (Component F) to assay buffer. Add 50 uL of prepared lysis buffer to the microplate wells. Incubate at room temperature for 10 min to allow cell lysis.
Note: If the cell extracts are prepared in culture media, we recommend running a medium control with the ß-galactosidase standard to establish the effects of the medium on assay performance.
ß-galactosidase substrate solution: Dilute ß-galactosidase substrate (Component A) 1:200 in assay buffer. For each experiment, prepare new substrate solution.
Prepare dilutions of ß-galactosidase standard (optional): Dilute ß-galactosidase (Component C) to 500 ng/mL (1:200) in assay buffer containing Triton X-100. Then make 3-fold serial dilutions to get concentration of 166.66, 55.55, 18.52, 6.17, 2.06, 0.686 ng/mL, include a blank control.
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文献和实验相关实验
范围较窄，灵敏性较低。 (2)β半乳糖苷酶：β半乳糖苷酶由大肠杆菌lacZ基因编码，可催化半乳糖苷水解。最大优势是易于用免疫组织化学法观测其原位表达，是最常用的监测转染率的报道基因之一。以邻―硝基苯―β―D―半乳吡喃糖苷(ONPG)为底物可用标准的比色法检测酶活性，其检测动力学范围为6个数量级。氯酚红―β―D―半乳吡喃糖苷(CPRG)是另一个可用比色法检测酶活性的底物，其灵敏度比ONPG高近10倍。以MUG和荧光素二半乳糖苷(FDG)为底物则可用荧光法检测其活性。此法可检测单个
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FDG ß-半乳糖苷酶检测试剂盒 荧光法
￥4462.50
