390 组织蛋白酶 D 检测试剂盒 荧光法、390 Cathepsin D Assay Kit *Fluorimetric*详细如下: 390 组织蛋白酶 D 活性检测试剂盒使用 Mca/Dnp FRET 肽来测量酶活性。在 FRET 肽中,Mca 的荧光被 Dnp 淬灭。在被组织蛋白酶 D 切割成两个单独的片段后,Mca 的荧光被恢复,并且可以在激发/发射 = 330 nm/390 nm 下进行监测。组织蛋白酶是一类球状溶酶体蛋白酶,在哺乳动物细胞更新中起着至关重要的作用。它们降解多肽并通过它们的底物特异性来区分。组织蛋白酶 D 是溶酶体天冬氨酸蛋白酶,在细胞内蛋白质分解中具有活性。 Add test compounds and diluted enzyme solution to the microplate wells. The suggested volume of enzyme solution for one well of a 96-well plate is 40 uL and test compound is 10 uL. Simultaneously establish the following control wells, as deemed necessary: Positive control contains the enzyme without test compound. Inhibitor control contains Cathepsin D enzyme and pepstatin. Vehicle control contains Cathepsin D enzyme and vehicle used in delivering test compound (e.g. DMSO, concentration not to exceed 1%). Test compound control contains assay buffer and test compound. Some test compounds have strong autofluorescence and may give false results. Substrate control contains assay buffer. Using the assay buffer, bring the total volume of all controls to 50 uL. Optional: Pre-incubate the plate for 10 min. at assay temperature. Any temperature (the assay temperature) from room temperature to 37℃ may be used, as long as the subsequent incubations are performed at the same temperature.