520 肾素检测试剂盒荧光检测、520 Renin Assay Kit *Fluorimetric*详细如下: 520 肾素检测试剂盒为高通量筛选肾素抑制剂和使用 5-FAM/QXL™520 荧光共振能量转移 (FRET) 肽连续检测肾素活性提供了一种方便的检测方法。在 FRET 肽中,5-FAM 的荧光被 QXL™520 淬灭。在被肾素切割成两个单独的片段后,5-FAM 的荧光被恢复,并且可以在激发/发射 = 490/520 nm 下进行监测。该检测比基于 EDANS/DABCYL 的检测灵敏约 50 倍,可检测 0.8 ng/ml 肾素。主持人:HEK293(哺乳动物细胞) Add test compounds and renin solution into the microplate wells. The suggested volume of renin solution for one well of a 96-well plate is 40 uL and test compound is 10 uL. Set up the following controls at the same time as deemed necessary: Positive control contains diluted renin without test compound. Inhibitor control contains diluted renin and diluted renin inhibitor (provided as undiluted in the kit). Vehicle control contains diluted renin and diluted vehicle used to deliver test compound (e.g. DMSO, concentration not to exceed 1%). Test compound control contains assay buffer and test compound. Some test compounds have strong autofluorescence and may give false results. Substrate control contains assay buffer. Note: Using the assay buffer, bring the total volume of all controls to 50 uL (96-well plate).