520 MMP-10 荧光检测试剂盒、520 MMP - 10 Assay Kit *Fluorimetric*详细如下: MMP-10 (stromelysin 2) 切割细胞外基质蛋白,但不能切割三螺旋纤维状胶原蛋白。这种酶涉及多种病理状况,例如癌症、关节炎和伤口愈合。520 MMP-10 Assay Kit 使用 5-FAM(荧光团)和 QXL™520(猝灭剂)标记的 FRET 肽底物来连续测量酶活性。在完整的 FRET 肽中,5-FAM 的荧光被 QXL™ 520 淬灭。在 MMP-10 切割 FRET 肽后,5-FAM 的荧光被恢复,并且可以在激发/发射时连续监测 = 490 纳米/520 纳米。具有优越的荧光量子产率和更长的发射波长,检测限:8 ng/ml Incubate pro-MMP-10 with 1 mM AP、MA (diluted Component C) for1 hour at 37°C. Activate pro-MMP-10 immediately before the experiment. Note 1: Keep activated enzyme on ice. Avoid vigorous vortex of the enzyme. Prolonged storage of activated enzyme will further de-activate the enzyme. Note 2: AP、MA can be diluted with assay buffer (Component D). AP、MA belongs to the organic mercury class of compounds and must be handled with care! Dispose according to appropriate regulations. Note 3: Activation of zymogen by AP、MA at higher protein concentration is preferred. After activation, the enzyme may be further diluted.