相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 技术资料
- 库存:
大量
- 英文名:
520 MMP - 10 Assay Kit *Fluorimetric*
- CAS号:
520 MMP - 10 Assay Kit *Fluorimetric*
- 保质期:
详见说明
- 供应商:
齐源生物
- 保存条件:
-20°C
- 规格:
1 kit
520 MMP-10 荧光检测试剂盒、520 MMP - 10 Assay Kit *Fluorimetric*详细如下:
MMP-10 (stromelysin 2) 切割细胞外基质蛋白,但不能切割三螺旋纤维状胶原蛋白。这种酶涉及多种病理状况,例如癌症、关节炎和伤口愈合。520 MMP-10 Assay Kit 使用 5-FAM(荧光团)和 QXL™520(猝灭剂)标记的 FRET 肽底物来连续测量酶活性。在完整的 FRET 肽中,5-FAM 的荧光被 QXL™ 520 淬灭。在 MMP-10 切割 FRET 肽后,5-FAM 的荧光被恢复,并且可以在激发/发射时连续监测 = 490 纳米/520 纳米。具有优越的荧光量子产率和更长的发射波长,检测限:8 ng/ml
Incubate pro-MMP-10 with 1 mM AP、MA (diluted Component C) for1 hour at 37°C. Activate pro-MMP-10 immediately before the experiment.
Note 1: Keep activated enzyme on ice. Avoid vigorous vortex of the enzyme. Prolonged storage of activated enzyme will further de-activate the enzyme.
Note 2: AP、MA can be diluted with assay buffer (Component D). AP、MA belongs to the organic mercury class of compounds and must be handled with care! Dispose according to appropriate regulations.
Note 3: Activation of zymogen by AP、MA at higher protein concentration is preferred. After activation, the enzyme may be further diluted.
MMP-10 (stromelysin 2) 切割细胞外基质蛋白,但不能切割三螺旋纤维状胶原蛋白。这种酶涉及多种病理状况,例如癌症、关节炎和伤口愈合。520 MMP-10 Assay Kit 使用 5-FAM(荧光团)和 QXL™520(猝灭剂)标记的 FRET 肽底物来连续测量酶活性。在完整的 FRET 肽中,5-FAM 的荧光被 QXL™ 520 淬灭。在 MMP-10 切割 FRET 肽后,5-FAM 的荧光被恢复,并且可以在激发/发射时连续监测 = 490 纳米/520 纳米。具有优越的荧光量子产率和更长的发射波长,检测限:8 ng/ml
Incubate pro-MMP-10 with 1 mM AP、MA (diluted Component C) for1 hour at 37°C. Activate pro-MMP-10 immediately before the experiment.
Note 1: Keep activated enzyme on ice. Avoid vigorous vortex of the enzyme. Prolonged storage of activated enzyme will further de-activate the enzyme.
Note 2: AP、MA can be diluted with assay buffer (Component D). AP、MA belongs to the organic mercury class of compounds and must be handled with care! Dispose according to appropriate regulations.
Note 3: Activation of zymogen by AP、MA at higher protein concentration is preferred. After activation, the enzyme may be further diluted.
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
技术资料暂无技术资料 索取技术资料
520 MMP-10 荧光检测试剂盒
¥6720









