BT-plex High Sensitivity Human Alzheimer’s Disease Biomarker Panel (5-plex) is a bead-based multiplex assay panel, using magnetic fluorescence-encoded beads for use with regular flow cytometers. The panel allows simultaneous quantification of Amyloid Beta 1-40 (Aßx40), Amyloid Beta 1-42 (Aßx42), Alpha-Synuclein (α-SYN), Total Tau, and Phosphorylated Tau at Threonine 181 site (pTau-181). The kit provides ultra-sensitive detection (fg/mL) and is more robust in analytical performance than similar multiplex assays on the market. This panel has been tested for use on cell culture supernatant, serum, plasma, and other body fluid samples.