520 MMP-12 荧光检测试剂盒、520 MMP-12 Assay Kit *Fluorimetric*详细如下: 基质金属蛋白酶 (MMP) 属于能够消化细胞外基质成分的分泌型或膜相关蛋白家族。MMP-12(巨噬细胞弹性蛋白酶)在活化的巨噬细胞中表达,可能在烟雾引起的肺气肿中起作用。 520 MMP-12 Assay Kit 使用 5-FAM(荧光团)和 QXL520™(猝灭剂)标记的 FRET 肽底物来连续测量酶活性。该底物对 MMP-12 表现出优异的特异性,与 MMP-1、2、7、8、9、9、13 和 14 的交叉反应最小。在完整的 FRET 肽中,5-FAM 的荧光被 QXL520™ 淬灭. 在 MMP-12 裂解 FRET 肽后,5-FAM 的荧光被恢复,并且可以在激发/发射 = 490 nm/520 nm 下连续监测。基于 5-FAM/QXL520™ 的 FRET 肽具有出色的荧光量子产率和更长的发射波长,较少受测试化合物和细胞成分的自发荧光干扰,并提供更好的检测灵敏度。检测限:10 ng/ml Add test compounds and MMP-12 diluent into microplate. The suggested total volume of MMP-12 diluent and test compound is 50uL/well. Simultaneously set up the following controls: Positive control contains MMP-12 diluent without test compound. Inhibitor control contains MMP-12 diluent and a known MMP-12 inhibitor. Vehicle control contains MMP-12 diluent and vehicle used in delivering test compound (e.g. DMSO). Test compound control contains assay buffer and test compound. Some test compounds have strong auto fluorescence and may give false results. Substrate control contains assay buffer only. Using the assay buffer (Component D), bring the total volume of all controls to 50uL/well. Incubate the plate at the desired temperature for enzymatic reaction for 10-15 min. Also incubate MMP-12 substrate solution at the same temperature.