490 MMP-12 检测试剂盒 荧光法、490 MMP-12 Assay Kit *Fluorimetric*详细如下: 基质金属蛋白酶 (MMP) 属于能够消化细胞外基质成分的分泌型或膜相关蛋白家族。MMP-12(巨噬细胞弹性蛋白酶)与许多疾病的病理学有关,例如肺气肿、主动脉病变和癌症。最近显示它在类风湿性关节炎的发病机制中起作用。490 MMP-12 检测试剂盒是一个完整的检测系统,旨在通过使用荧光 MMP-12 底物连续分析 MMP-12 活性或筛选 MMP-12 抑制剂。 吸光度 (nm):340、发射(纳米):490、检测限:43 ng/ml Add test compounds and MMP-12 diluent into microplate. The suggested total volume of MMP-12 diluent and test compound is 50uL/well. Positive control contains MMP-12 diluent without test compound. Inhibitor control contains MMP-12 diluent and a known MMP-12 inhibitor. Vehicle control contains MMP-12 diluent and vehicle used in delivering test compound (e.g. DMSO). Test compound control contains assay buffer and test compound. Some test compounds have strong autofluorescence and may give false results. Note: Use assay buffer (Component D) to bring the total volume of all the controls to 50uL/well. Incubate the plate at the desired temperature for enzymatic reaction (e.g. 25℃ or 37℃) for 10-15 min. In the mean time, also incubate MMP-12 substrate solution at the same temperature. Add 50uL/well of MMP-12 substrate solution. Mix the reagents completely by shaking the plate gently for 30-60 second. For kinetic reading: Immediately start measuring fluorescence intensity at Ex/Em=340±30 nm/490±30 nm continuously and record data every 5 minutes for 30 to 60 minutes. For end-point reading: Incubate the reaction at room temperature for 30 to 60 minutes. Keep the plate from direct light. Optional: Add 50 L/well stop solution (Component E). Mix the reagents. Then measure fluorescence intensity at Ex/Em=340±30 nm/490±0 nm.