490 MMP-13 检测试剂盒 荧光法、490 MMP-13 Assay Kit *Fluorimetric*详细如下: 基质金属蛋白酶 (MMP) 属于能够消化细胞外基质成分的分泌型或膜相关蛋白家族。MMP-13(collagenase-3,大鼠胶原酶)与关节炎、哮喘等多种疾病有关。 490 MMP-13 检测试剂盒是一个完整的检测系统,旨在通过使用荧光 MMP-13 底物持续分析 MMP-13 活性或筛选 MMP-13 抑制剂。 MMP 家族成员的底物序列特异性较差,因此很难单独使用肽底物来区分特定 MMP 与其他 MMP 的活性。如果您的样品中同时存在多种 MMP,并且您想专门测量 MMP-13 活性,请选择 Plus MMP-13 检测试剂盒,Cat#72019。吸光度 (nm):340、发射(纳米):490 Incubate pro-MMP-13 with 1 mM AP、MA (diluted Component C) for 40 min at 37°C. Activate pro-MMP-13 immediately before the experiment. Note 1: Keep activated enzyme on ice. Avoid vigorously vortexing the enzyme. Prolonged storage of activated enzyme will further de-activate the enzyme. Note 2: AP、MA can be diluted with assay buffer (Component D). AP、MA belongs to organic mercury. Handle with care! Dispose it according to appropriate regulations. Note 3: It is preferred that the zymogen is activated by AP、MA at higher protein concentration. After activation, you may dilute the enzyme for further experiment Add test compounds and MMP-13 diluent into microplate. The suggested total volume of MMP-13 diluent and test compound is 50uL. Positive control contains MMP-13 diluent without test compound. Inhibitor control contains MMP-13 diluent and known MMP-13 inhibitor. Vehicle control contains MMP-13 diluent and vehicle used to deliver test compound (e.g. DMSO). Test compound control contains assay buffer and test compound. Some test compounds have strong autofluorescence and may give false results. Substrate control contains assay buffer only. Note: Match the total volume of all the controls to 50uL/well by assay buffer.