490 MMP-9 荧光检测试剂盒、490 MMP-9 Assay Kit *Fluorimetric*详细如下: 基质金属蛋白酶 (MMP) 属于能够消化细胞外基质成分的分泌型或膜相关蛋白家族。MMP-9(gelatinase-B,collagenase-IV)与癌症、血管生成、脱发和转移等多种疾病有关。 SensoLyte® 490 MMP-9 检测试剂盒是一个完整的检测系统,旨在持续分析 MMP 活性或使用荧光 MMP-9 底物筛选 MMP-9 抑制剂。检测以方便的 96 孔或 384 孔微孔板形式进行。该试剂盒为 MMP-9 分析提供了速度、便利性和准确性。 MMP 家族成员的底物序列特异性较差,因此很难单独使用肽底物来区分特定 MMP 与其他 MMP 的活性。如果您的样品中同时存在多种 MMP,并且您想专门测量 MMP-9 活性,请选择 Plus MMP-9 检测试剂盒,Cat#72017。吸光度 (nm):340、发射(纳米):490 Add test compounds and MMP-9 diluent into microplate. The suggested total volume of MMP-9 diluent and test compound is 50uL/well. Positive control contains MMP-9 diluent without test compound. Inhibitor control contains MMP-9 diluent and a known MMP-9 inhibitor. Vehicle control contains MMP-9 diluent and vehicle used in delivering test compound (e.g. DMSO). Test compound control contains assay buffer and test compound. Some test compounds have strong auto fluorescence and may give false results. Substrate control contains assay buffer only. Note: Use assay buffer (Component D) to bring the total volume of all the controls to 50uL/well. Incubate the plate at the desired temperature for enzymatic reaction (e.g. 25℃ or 37℃) for 10-15 min. In the mean time, also incubate MMP-9 substrate solution at the same temperature. Add 50uL of MMP-9 substrate solution into the wells. Mix the reagents completely by shaking the plate gently for 30-60 second. For kinetic reading: Immediately start measuring fluorescence intensity at Ex/Em=340±30 nm/490±30 nm continuously and record data every 5 minutes for 30 to 60 minutes. For end-point reading: Incubate the reaction at room temperature for 30 to 60 minutes. Keep the plate from direct light. Optional: Add 50uL/well stop solution (Component E). Mix the reagents. Then measure fluorescence intensity at Ex/Em=340±30 nm/490±30 nm.