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- 详细信息
- 技术资料
- 库存：
大量
- 英文名：
490 MMP-8 Assay Kit *Fluorimetric*
- CAS号：
490 MMP-8 Assay Kit *Fluorimetric*
- 保质期：
详见说明
- 供应商：
齐源生物
- 保存条件：
-20°C
- 规格：
1 kit
490 MMP-8 荧光检测试剂盒、490 MMP-8 Assay Kit *Fluorimetric*详细如下：
基质金属蛋白酶 (MMP) 属于能够消化细胞外基质成分的分泌型或膜相关蛋白家族。MMP-8（collagenase-2，中性粒细胞胶原酶）与癌症、关节炎和哮喘等多种疾病有关。
490 MMP-8 检测试剂盒是一个完整的检测系统，旨在通过使用荧光 MMP-8 底物连续分析 MMP 活性或筛选 MMP-8 抑制剂。吸光度 (nm)：340、发射（纳米）：490
Incubate pro-MMP-8 with 1 mM AP、MA (diluted Component C) for 1 hr at 37°C.
Activate pro-MMP-8 immediately before the experiment.
Note 1: Keep activated enzyme on ice. Avoid vigorous vortex of the enzyme. Prolonged storage of
activated enzyme will further de-activate the enzyme.
Note 2: AP、MA can be diluted with assay buffer (Component D). AP、MA belongs to organic mercury.
Handle with care! Dispose it according to appropriate regulations.
Note 3: Activate zymogen by AP、MA at higher protein concentration. After activation, the enzyme may be further diluted.
Add test compounds and MMP-8 diluent into microplate. The suggested total volume
of MMP-8 diluent and test compound is 50uL/well
Simultaneously set up the following controls.
Positive control contains MMP-8 diluent without test compound.
Inhibitor control contains MMP-8 diluent and a known MMP-8 inhibitor.
Vehicle control contains MMP-8 diluent and vehicle used in delivering test compound (e.g. DMSO).
Test compound control contains assay buffer and test compound. Some test
compounds have strong auto fluorescence and may give false results.
Substrate control contains assay buffer only.
Note: Use assay buffer (Component D) to bring the total volume of all the controls to 50uL/well.
Incubate the plate at the desired temperature for enzymatic reaction (e.g. 25℃ or 37℃)
for 10-15 min. In the mean time, also incubate MMP-8 substrate solution at the same temperature.
Add 50uL of MMP-8 substrate solution into the wells. Mix the reagents completely by
shaking the plate gently for 30-60 second.
For kinetic reading: Immediately start measuring fluorescence intensity at
Ex/Em=340±30 nm/490±30 nm continuously and record data every 5 minutes for 30 to 60 minutes.
For end-point reading: Incubate the reaction at room temperature for 30 to 60 minutes.
Keep the plate from direct light. Optional: Add 50uL/well stop solution (Component
E). Mix the reagents. Then measure fluorescence intensity at Ex/Em=340±30 nm/490±30 nm.
基质金属蛋白酶 (MMP) 属于能够消化细胞外基质成分的分泌型或膜相关蛋白家族。MMP-8（collagenase-2，中性粒细胞胶原酶）与癌症、关节炎和哮喘等多种疾病有关。
490 MMP-8 检测试剂盒是一个完整的检测系统，旨在通过使用荧光 MMP-8 底物连续分析 MMP 活性或筛选 MMP-8 抑制剂。吸光度 (nm)：340、发射（纳米）：490
Incubate pro-MMP-8 with 1 mM AP、MA (diluted Component C) for 1 hr at 37°C.
Activate pro-MMP-8 immediately before the experiment.
Note 1: Keep activated enzyme on ice. Avoid vigorous vortex of the enzyme. Prolonged storage of
activated enzyme will further de-activate the enzyme.
Note 2: AP、MA can be diluted with assay buffer (Component D). AP、MA belongs to organic mercury.
Handle with care! Dispose it according to appropriate regulations.
Note 3: Activate zymogen by AP、MA at higher protein concentration. After activation, the enzyme may be further diluted.
Add test compounds and MMP-8 diluent into microplate. The suggested total volume
of MMP-8 diluent and test compound is 50uL/well
Simultaneously set up the following controls.
Positive control contains MMP-8 diluent without test compound.
Inhibitor control contains MMP-8 diluent and a known MMP-8 inhibitor.
Vehicle control contains MMP-8 diluent and vehicle used in delivering test compound (e.g. DMSO).
Test compound control contains assay buffer and test compound. Some test
compounds have strong auto fluorescence and may give false results.
Substrate control contains assay buffer only.
Note: Use assay buffer (Component D) to bring the total volume of all the controls to 50uL/well.
Incubate the plate at the desired temperature for enzymatic reaction (e.g. 25℃ or 37℃)
for 10-15 min. In the mean time, also incubate MMP-8 substrate solution at the same temperature.
Add 50uL of MMP-8 substrate solution into the wells. Mix the reagents completely by
shaking the plate gently for 30-60 second.
For kinetic reading: Immediately start measuring fluorescence intensity at
Ex/Em=340±30 nm/490±30 nm continuously and record data every 5 minutes for 30 to 60 minutes.
For end-point reading: Incubate the reaction at room temperature for 30 to 60 minutes.
Keep the plate from direct light. Optional: Add 50uL/well stop solution (Component
E). Mix the reagents. Then measure fluorescence intensity at Ex/Em=340±30 nm/490±30 nm.
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490 MMP-8 荧光检测试剂盒
￥9282
