Anti-Human MOG (1-125) Mouse IgG Specific ELISA Kit
CAS号:
Anti-Human MOG (1-125) Mouse IgG Specific ELISA Kit
保质期:
详见说明
供应商:
齐源生物
保存条件:
-20°C
规格:
1 kit
抗人MOG(1-125)小鼠Ig G特异性ELISA试剂盒比色法、Anti-Human MOG (1-125) Mouse IgG Specific ELISA Kit详细如下: 该试剂盒经过优化,可检测小鼠血清或脑脊液中的抗人 MOG (1-125) IgG。孔用重组人 MOG (1-125) 蛋白预包被并用 BSA 预封闭。使用 ELISA 对血清或脑脊液中抗人 MOG IgG 的量进行量化。包括小鼠抗人 MOG IgG 标准品。提供充足的材料和试剂,以在 96 孔板格式中进行 96 次测定。检测限:1纳克/毫升、主持人:大肠杆菌 1 Establish dilution range of serum samples: Serial dilutions of serum samples can start from 1:1k, 1:5k, 1:25k, 1:125k. Use 1X Sample Dilution Buffer (Component C) to do the dilution (an example is shown in Table 1). Depending on the amount of antibody present, the dilution range can be further adjusted. 2 Arrange and label strips (Component A) based on the number of wells with standard and samples. An example is shown in Table 1. Although diluted standard and samples can be run as single points, duplicates are recommended. 3 Dilute anti-human MOG (1-125) IgG standard (Component B) with 1X Sample Dilution Buffer (Component C) according to the Table 2. 4 Add 100 μl of the diluted standards and 100 μl of 1X Sample Dilution Buffer (Component C) as a blank into appropriate wells. 5 Add diluted samples into appropriate wells (depends on the number of samples to be tested). After adding the standards and samples to the wells, cover the plate and incubate at room temperature for 60 min with gentle shaking. 6 Prepare 1X working wash buffer by diluting the 10X Wash Buffer (Component D) with di H2O. 7 Wash wells five times at 250 μl/well of 1X washing buffer. Pat dry. 8 Dilute goat anti-Mouse IgG-HRP (Component G) secondary antibody (2nd Ab) with Sample Dilution Buffer (Component C): working solution at 1:2,000 dilution . Add 100 μl of the diluted 2nd Ab into each well; incubate plate at room temperature for 45-60 min with gentle shaking. 9 Wash wells five times with 200 μl per well of 1X wash buffer. Pat dry. Clean the outside bottom of the wells with lens paper if necessary before the next step (this ensures accurate absorbance reading). 10 Add 100 μl of the TMB color substrate solution (Component E) into each well. Tap plate gently and incubate at room temperature until blue gradient is clearly observed across the wells (1-15 min). It may be necessary to adjust color development time so that absorbance values are within the detection range. 11 Add 50 μl of the Stop Solution (Component F) into each well and tap plate gently (blue color will turn to yellow). Measure absorbance (OD) at 450 nm using a microplate absorbance reader within 20 minutes after adding stop solution.