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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
Anti-Human β-Amyloid (1-40) Quantitative ELISA *Colorimetric*
- CAS号：
Anti-Human β-Amyloid (1-40) Quantitative ELISA *Colorimetric*
- 保质期：
详见说明
- 供应商：
齐源生物
- 保存条件：
-80°C
- 规格：
1 kit
抗-人 β-淀粉样蛋白 (1-40) 定量 ELISA 试剂盒 *比色法*、Anti-Human β-Amyloid (1-40) Quantitative ELISA *Colorimetric*详细如下：
这款 SensoLyte® 高灵敏度 (2 pg/ml) β-淀粉样蛋白 (1-40) 定量 ELISA 试剂盒（人）提供了一种方便的定量测定方法，用于确定人脑中的人 β-淀粉样蛋白 (1-40) (Aβ40) 量裂解物、转基因小鼠脑裂解物、人脑脊液、血浆或唾液。孔预涂有单克隆抗β-淀粉样蛋白 (1-40) 特异性捕获抗体，并用专有的封闭溶液封闭。人 β-淀粉样蛋白 (1-40) 的量使用 ELISA 进行量化。提供充足的材料和试剂来进行 96 次检测。
检测方法：比色法
检测限：2 pg/ml
来源/物种：人
This kit is optimized to detect human beta-Amyloid (1-40) peptide in human brain lysate, transgenic mouse
brain lysate, human cerebrospinal fluid, plasma, or saliva. Wells are pre-coated with monoclonal anti-betaAmyloid (1-40) specific capture antibodies and blocked with a proprietary blocking solution. The amount of
human beta-Amyloid (1-40) is quantified using ELISA. Ample materials and reagents are provided to
perform 96 assays.
• Convenient Format
o Pre-coated and pre-blocked 96-well strip plate
o Ready-to-use substrate solution and other assay components
o One step assay (samples and detection antibodies are added simultaneously)
o 1 hour assay time at room temperature (excluding incubation)
• High Sensitivity
o Detects as low as 2 pg/ml (13 fmoles/ml) of human beta-Amyloid (1-40)
• Broad Dynamic Range
o 3.9-250 pg/ml of human beta-Amyloid (1-40) peptide
This kit is optimized to detect human beta-Amyloid (1-40) peptide in human brain lysate, transgenic mouse
brain lysate, human cerebrospinal fluid, plasma, or saliva. Wells are pre-coated with monoclonal anti-betaAmyloid (1-40) specific capture antibodies and blocked with a proprietary blocking solution. The amount of
human beta-Amyloid (1-40) is quantified using ELISA. Ample materials and reagents are provided to
perform 96 assays.
• Convenient Format
o Pre-coated and pre-blocked 96-well strip plate
o Ready-to-use substrate solution and other assay components
o One step assay (samples and detection antibodies are added simultaneously)
o 1 hour assay time at room temperature (excluding incubation)
• High Sensitivity
o Detects as low as 2 pg/ml (13 fmoles/ml) of human beta-Amyloid (1-40)
• Broad Dynamic Range
o 3.9-250 pg/ml of human beta-Amyloid (1-40) peptide
Add 100 μl per well of the diluted Aβ40 standards (Starting with Step 3 from 1.3) in duplicates including blank.
We recommend diluting plasma at 1:20 ratio and CSF at 1:4 ratio with 1 X Sample Dilution Buffer to avoid
sample matrix effects. For transgenic mouse brain lysate it is recommended to use 1:100 dilution ratio for young
mice (5-10 weeks old) and 1:400 dilution ration for older mice (10-15 weeks old). In addition, Protease inhibitor
cocktail with PMSF must be added to all samples to avoid protein degradation (a recipe example is provided in
the Appendix section).
Add 100 μl of the diluted samples into appropriate wells.
Add 50 μl of the diluted detection antibody solution (from Step 1.4) into each well to be assayed, apply
Adhesive Plate Cover (Component J), and incubate plate overnight at 4°C. Protect wells from the light.
Prepare 1X working wash buffer by diluting the 10 X Wash Buffer (Component D) with deionized H2O.
After plate incubation, aspirate the wells and wash them with 350 μl/well of 1x wash buffer 6-7 times. Allow
20 seconds soaking time before emptying the wells between washes. Pat dry the plate using a paper towel and
wipe clean outside of wells with non-abrasive paper to ensure accurate optical reading.
Insufficient washing will increase background reading.
Add 100 μl of the TMB Color Substrate Solution (Component E) into each well. Tap plate gently and incubate at
room temperature until blue gradient is clearly observed across the wells (5- 15 min). It may be necessary to
adjust color development time so that absorbance values will fall within the detection range. Samples may
require further dilution if Aβ40 concentration is too high.
Add 50 μl of the Stop Solution (Component F) into each well and tap plate gently (blue color will turn to yellow).
Measure absorbance (OD) at 450 nm using a microplate absorbance reader within 20 minutes after adding the
Stop Solution.
Calculate the concentration of Aβ40 in samples.
Determine the average values (if replicates are used) for the Aβ40 standard and sample absorbance readings.
Plot calibration curve using Linear Regression curve-fit. R2 should be higher than 0.98. There should be at
least 5 standard concentrations in the calculation to ensure statistical significance. The reading for the highest
standard concentration (250 pg/ml) can be excluded from the curve if signal is too strong.
Choose absorbance values for the diluted samples that are within the range used in the Aβ40 standard
curve, and calculate the concentration of human Aβ40 in the tested sample(s). Calculated concentrations must
be multiplied by the sample dilution factor.
Typical Aβ40 Standard Curve:
Please note, new standard curve must be generated each time when the assay is run.
这款 SensoLyte® 高灵敏度 (2 pg/ml) β-淀粉样蛋白 (1-40) 定量 ELISA 试剂盒（人）提供了一种方便的定量测定方法，用于确定人脑中的人 β-淀粉样蛋白 (1-40) (Aβ40) 量裂解物、转基因小鼠脑裂解物、人脑脊液、血浆或唾液。孔预涂有单克隆抗β-淀粉样蛋白 (1-40) 特异性捕获抗体，并用专有的封闭溶液封闭。人 β-淀粉样蛋白 (1-40) 的量使用 ELISA 进行量化。提供充足的材料和试剂来进行 96 次检测。
检测方法：比色法
检测限：2 pg/ml
来源/物种：人
This kit is optimized to detect human beta-Amyloid (1-40) peptide in human brain lysate, transgenic mouse
brain lysate, human cerebrospinal fluid, plasma, or saliva. Wells are pre-coated with monoclonal anti-betaAmyloid (1-40) specific capture antibodies and blocked with a proprietary blocking solution. The amount of
human beta-Amyloid (1-40) is quantified using ELISA. Ample materials and reagents are provided to
perform 96 assays.
• Convenient Format
o Pre-coated and pre-blocked 96-well strip plate
o Ready-to-use substrate solution and other assay components
o One step assay (samples and detection antibodies are added simultaneously)
o 1 hour assay time at room temperature (excluding incubation)
• High Sensitivity
o Detects as low as 2 pg/ml (13 fmoles/ml) of human beta-Amyloid (1-40)
• Broad Dynamic Range
o 3.9-250 pg/ml of human beta-Amyloid (1-40) peptide
This kit is optimized to detect human beta-Amyloid (1-40) peptide in human brain lysate, transgenic mouse
brain lysate, human cerebrospinal fluid, plasma, or saliva. Wells are pre-coated with monoclonal anti-betaAmyloid (1-40) specific capture antibodies and blocked with a proprietary blocking solution. The amount of
human beta-Amyloid (1-40) is quantified using ELISA. Ample materials and reagents are provided to
perform 96 assays.
• Convenient Format
o Pre-coated and pre-blocked 96-well strip plate
o Ready-to-use substrate solution and other assay components
o One step assay (samples and detection antibodies are added simultaneously)
o 1 hour assay time at room temperature (excluding incubation)
• High Sensitivity
o Detects as low as 2 pg/ml (13 fmoles/ml) of human beta-Amyloid (1-40)
• Broad Dynamic Range
o 3.9-250 pg/ml of human beta-Amyloid (1-40) peptide
Add 100 μl per well of the diluted Aβ40 standards (Starting with Step 3 from 1.3) in duplicates including blank.
We recommend diluting plasma at 1:20 ratio and CSF at 1:4 ratio with 1 X Sample Dilution Buffer to avoid
sample matrix effects. For transgenic mouse brain lysate it is recommended to use 1:100 dilution ratio for young
mice (5-10 weeks old) and 1:400 dilution ration for older mice (10-15 weeks old). In addition, Protease inhibitor
cocktail with PMSF must be added to all samples to avoid protein degradation (a recipe example is provided in
the Appendix section).
Add 100 μl of the diluted samples into appropriate wells.
Add 50 μl of the diluted detection antibody solution (from Step 1.4) into each well to be assayed, apply
Adhesive Plate Cover (Component J), and incubate plate overnight at 4°C. Protect wells from the light.
Prepare 1X working wash buffer by diluting the 10 X Wash Buffer (Component D) with deionized H2O.
After plate incubation, aspirate the wells and wash them with 350 μl/well of 1x wash buffer 6-7 times. Allow
20 seconds soaking time before emptying the wells between washes. Pat dry the plate using a paper towel and
wipe clean outside of wells with non-abrasive paper to ensure accurate optical reading.
Insufficient washing will increase background reading.
Add 100 μl of the TMB Color Substrate Solution (Component E) into each well. Tap plate gently and incubate at
room temperature until blue gradient is clearly observed across the wells (5- 15 min). It may be necessary to
adjust color development time so that absorbance values will fall within the detection range. Samples may
require further dilution if Aβ40 concentration is too high.
Add 50 μl of the Stop Solution (Component F) into each well and tap plate gently (blue color will turn to yellow).
Measure absorbance (OD) at 450 nm using a microplate absorbance reader within 20 minutes after adding the
Stop Solution.
Calculate the concentration of Aβ40 in samples.
Determine the average values (if replicates are used) for the Aβ40 standard and sample absorbance readings.
Plot calibration curve using Linear Regression curve-fit. R2 should be higher than 0.98. There should be at
least 5 standard concentrations in the calculation to ensure statistical significance. The reading for the highest
standard concentration (250 pg/ml) can be excluded from the curve if signal is too strong.
Choose absorbance values for the diluted samples that are within the range used in the Aβ40 standard
curve, and calculate the concentration of human Aβ40 in the tested sample(s). Calculated concentrations must
be multiplied by the sample dilution factor.
Typical Aβ40 Standard Curve:
Please note, new standard curve must be generated each time when the assay is run.
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抗-人 β-淀粉样蛋白 (1-40) 定量 ELISA 试剂盒 *比色法*
￥7056
