- Bifidobacterium-mediated high-intensity focused ultrasound for solid tumor therapy: comparison of two nanoparticle delivery methods
引用内容:lStreptavidin B. longum antibody was purchased from Wuhan AtaGenix Laboratories Ltd., Co.(Wuhan, China)
- Evaluation of differentially expressed microRNAs in vitrified oocytes by next generation sequencing
引用内容:After transfer, membranes were blocked in TBST containing 5% (w/v) non-fat powdered milk for 1h, and then incubated with the primary antibodies against PTEN (1:1000, Proteintech Group, Wuhan, China) and GAPDH (1:3000, Atagenix, Wuhan, China) overnight at 4℃
- Activated PI3K/AKT Signaling Pathway Associates with Oxidative Stress and Impaired Developmental Potential of Vitrified-Thawed Oocytes
引用内容:AKT(1:1000, Affinity Biosciences, OH, USA), P-AKT (Ser473,1:1000, Affinity Biosciences, OH, USA), SIRT3 (1:1000,Proteintech Group, Wuhan, China), and GAPDH (1:3000,Atagenix, Wuhan, China), overnight at 4 °C
- Inhibiting SLC26A4 reverses cardiac hypertrophy in H9C2 cells and in rats
引用内容:lGAPDH (1/5,000, Atagenix, Wuhan, China)
- Protein profile of Dabry’s Sturgeon (Acipenser dabryanus) spermatozoa and relationship to sperm quality
引用内容:After blocking with 5% non-fat milk at room temperature for 2 h, the membranes were incubated with each primary antibodies,respectively, overnight at 4 ºC [anti-CKMT1A 1:400 (Atagenix); ATApla8239, rabbit anti-LDHB 1:400 (Atagenix); ATApla5470, rabbit; anti-MDH2 1:400 (Atagenix); ATApla1906,rabbit; GAPDH 1:5000 (Proteintech) 10494-1-AP, rabbit].
- Nobiletin selectively inhibits oral cancer cell growth by promoting apoptosis and DNA damage in vitro
引用内容:and anti-PARP, anti-capase-3,anti-gH2 AX (AtaGenix, Wuhan, China) was purchased from Wuhan Yipu Biological Technology Co.,Ltd.
- Variability in the protein profiles in spermatozoa of two sturgeon species
引用内容:After blocking with 5% non-fat milk at room temperature for 2 h, the membrane was incubated with primary antibody overnight at4˚C, (i.e., anti-LDHB 1:400; Atagenix, ATApla5470, rabbit; anti-CKMT1 1:400, Atagenix,ATApla8239, rabbit; or anti-β-actin 1:5000, Atagenix, ATA10167, mouse)
- Constitutive activation of STAT3 and cyclin D1 overexpression contribute to proliferation,migration and invasion in gastric cancer cells
引用内容:Each membrane was then incubated with various primary antibodies overnight at 4°C,including anti-pSTAT3 (1:1000, CST, 8204, rabbit), anti-STAT3 (1:1000, AtaGenix, ata17461,rabbit), anti-CCND1 (1:1000, AtaGenix, ata112-
63, rabbit), anti-GAPDH (1:5000, AtaGenix,ata17456, rabbit) at 4°C overnight. After washing for five times with TBST, the membrane was incubated with the corresponding secondary antibody conjugated with HRP (1:5000, AtaGenix, ata17817) for 1 h at room temperature.GAPDH was used for the internal control.
- 11 β ‑hydroxysteroid dehydrogenase‑1 is associated with the activation of hepatic stellate cells in the development of hepatic fibrosis
引用内容:The anti‑11 β ‑HSD1 antibody was purchased from AtaGenix (cat. no. ATA24000; 1:200)