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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
Purified anti-mouse Galectin-9 Antibody
- CAS号：
Purified anti-mouse Galectin-9 Antibody
- 保质期：
详见说明
- 供应商：
齐源生物
- 保存条件：
2-8℃
- 规格：
50 ug/ 500 ug
| 规格： | 50 ug | 产品价格： | ￥1700.0 |
|---|---|---|---|
| 规格： | 500 ug | 产品价格： | ￥7300.0 |
Purified 抗小鼠 Galectin-9 抗体、Purified anti-mouse Galectin-9 Antibody详细如下：
Description：
Galectin-9 is a mammalian lectin with a molecular weight of 40 kD that has two conserved carbohydrate recognition domains (CRDs) and forms homodimers. It recognizes N-acetyllactosamine (Galbeta1-4GlcNAc) and T-antigen (Galbeta1-3GalNAc); Tim-3 has been reported as its ligand. Galectin-9 is expressed by lymphocytes, dendritic cells, granulocytes, eosinophils, astrocytes, endothelial cells, fibroblasts and thymus epithelial cells. It may be retained intracellularly or be transported to the cell surface where it is cleaved, thereby generating a soluble form. Galectin-9 is involved in events such as cell aggregation, cell adhesion, chemotaxis and apoptosis. Importantly for the regulation of the immune response, Galectin-9 induces regulatory T cells and suppresses Th1 and Th17 responses.
Other Names：Lgals9, Gal-9, Ecalectin
Isotype：Rat IgG2a, κ
Isotype Control：Purified Rat IgG2a, κ Isotype Ctrl
Reactivity：Mouse
Antibody Type：Monoclonal
Host Species：Rat
Immunogen：Full length recombinant mouse Galectin-9 (M-type)
Formulation：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation：The antibody was purified by affinity chromatography.
Concentration：0.5 mg/ml
Storage & Handling：The antibody solution should be stored undiluted between 2°C and 8°C.
Application：
FC - Quality tested
ICFC, IHC-F, WB - Verified
ELISA - Reported in the literature, not verified in house
Recommended Usage：
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunohistochemical staining on frozen tissue sections, the suggested use is 5-10 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes：
Clone 108A2 recognizes the linker peptide of mouse Galectin-9; it does not cross-react with stable Galectin-9 (which is biologically active, genetically engineered, linker-less Galectin-9)3. Additional reported applications (for the relevant formats) include: sandwich ELISA as capture antibody1 and immunohistochemical staining of paraffin2 embedded tissue sections.
Application References：
Tsuboi Y, et al. 2007. Clin. Immunol. 124:221. (ELISA)
Personal communication. (IHC-Paraffin)
Oomizu S, et al. 2012. PLoS One. 7:e48574. PubMed
Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
Product Citations：
Oomizu S, et al. 2012. PLoS One. 7:e48574. PubMed
Sudhakar JN, et al. 2020. Nat Commun. 3.434722222. PubMed
Baloche V, et al. 2021. Scientific Reports. 11(1):5227. PubMed
Structure：Homodimer, each chain is a 343 aa protein of 40 kD that has two conserved carbohydrate recognition domains (CRDs)
Distribution：Lymphocytes, dendritic cells, neutrophils, eosinophils, astrocytes, endothelial cells, fibroblasts, thymus stromal/epithelial cells
Function：Cell aggregation, cell adhesion, chemotaxis, apoptosis, suppression of Th1 and Th17 responses, induction of regulatory T cells
Ligand/Receptor：Tim-3
Cell Type：Astrocytes, Dendritic cells, Endothelial cells, Eosinophils, Epithelial cells, Fibroblasts, Lymphocytes, Neutrophils, Tregs
Biology Area：Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family：Adhesion Molecules, Immune Checkpoint Receptors
Antigen References：
1. Klibi J, et al. 2009. Blood 113:1957.
2. Seki M, et al. 2008. Clin. Immunol. 127:78.
3. Tsuboi Y, et al. 2007. Clin. Immunol. 124:221.
4. Zhu C, et al. 2005. Nat. Immunol. 6:1245.
5. Dunphy JL, et al. 2002. J. Biol. Chem. 277:14916.
Description：
Galectin-9 is a mammalian lectin with a molecular weight of 40 kD that has two conserved carbohydrate recognition domains (CRDs) and forms homodimers. It recognizes N-acetyllactosamine (Galbeta1-4GlcNAc) and T-antigen (Galbeta1-3GalNAc); Tim-3 has been reported as its ligand. Galectin-9 is expressed by lymphocytes, dendritic cells, granulocytes, eosinophils, astrocytes, endothelial cells, fibroblasts and thymus epithelial cells. It may be retained intracellularly or be transported to the cell surface where it is cleaved, thereby generating a soluble form. Galectin-9 is involved in events such as cell aggregation, cell adhesion, chemotaxis and apoptosis. Importantly for the regulation of the immune response, Galectin-9 induces regulatory T cells and suppresses Th1 and Th17 responses.
Other Names：Lgals9, Gal-9, Ecalectin
Isotype：Rat IgG2a, κ
Isotype Control：Purified Rat IgG2a, κ Isotype Ctrl
Reactivity：Mouse
Antibody Type：Monoclonal
Host Species：Rat
Immunogen：Full length recombinant mouse Galectin-9 (M-type)
Formulation：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation：The antibody was purified by affinity chromatography.
Concentration：0.5 mg/ml
Storage & Handling：The antibody solution should be stored undiluted between 2°C and 8°C.
Application：
FC - Quality tested
ICFC, IHC-F, WB - Verified
ELISA - Reported in the literature, not verified in house
Recommended Usage：
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunohistochemical staining on frozen tissue sections, the suggested use is 5-10 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes：
Clone 108A2 recognizes the linker peptide of mouse Galectin-9; it does not cross-react with stable Galectin-9 (which is biologically active, genetically engineered, linker-less Galectin-9)3. Additional reported applications (for the relevant formats) include: sandwich ELISA as capture antibody1 and immunohistochemical staining of paraffin2 embedded tissue sections.
Application References：
Tsuboi Y, et al. 2007. Clin. Immunol. 124:221. (ELISA)
Personal communication. (IHC-Paraffin)
Oomizu S, et al. 2012. PLoS One. 7:e48574. PubMed
Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
Product Citations：
Oomizu S, et al. 2012. PLoS One. 7:e48574. PubMed
Sudhakar JN, et al. 2020. Nat Commun. 3.434722222. PubMed
Baloche V, et al. 2021. Scientific Reports. 11(1):5227. PubMed
Structure：Homodimer, each chain is a 343 aa protein of 40 kD that has two conserved carbohydrate recognition domains (CRDs)
Distribution：Lymphocytes, dendritic cells, neutrophils, eosinophils, astrocytes, endothelial cells, fibroblasts, thymus stromal/epithelial cells
Function：Cell aggregation, cell adhesion, chemotaxis, apoptosis, suppression of Th1 and Th17 responses, induction of regulatory T cells
Ligand/Receptor：Tim-3
Cell Type：Astrocytes, Dendritic cells, Endothelial cells, Eosinophils, Epithelial cells, Fibroblasts, Lymphocytes, Neutrophils, Tregs
Biology Area：Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family：Adhesion Molecules, Immune Checkpoint Receptors
Antigen References：
1. Klibi J, et al. 2009. Blood 113:1957.
2. Seki M, et al. 2008. Clin. Immunol. 127:78.
3. Tsuboi Y, et al. 2007. Clin. Immunol. 124:221.
4. Zhu C, et al. 2005. Nat. Immunol. 6:1245.
5. Dunphy JL, et al. 2002. J. Biol. Chem. 277:14916.
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Purified 抗小鼠 Galectin-9 抗体
￥1700 - 7300
