Purified anti-小鼠 CD45.2 抗体、Purified anti-mouse CD45.2 Antibody详细如下: Description: CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1. Other Names:Ly-5.2, LCA Isotype:Mouse (SJL) IgG2a, κ Isotype Control:Purified Mouse IgG2a, κ Isotype Ctrl Reactivity:Mouse Antibody Type:Monoclonal Host Species:Mouse Immunogen:B10.S mouse thymocytes and splenocytes Formulation:Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Preparation:The antibody was purified by affinity chromatography. Concentration:0.5 mg/ml Storage & Handling:The antibody solution should be stored undiluted between 2°C and 8°C. Application:FC - Quality tested、Block, IHC-F, IP - Reported in the literature, not verified in house Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Application Notes: The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3. Application References: Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block) Yakura H, et al. 1986. J. Immunol. 136:2729. (Block) Suzuki K, et al. 2000. Immunity 13:691. (IHC) Shen FW, et al. 1986. Immunogenetics 24:146. (IP) Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837. Pascal V, et al. 2007. J. Immunol. 179:1751. Burman AC, et al. 2007. Blood 110:1064. Kincaid EZ, et al. 2007. J. Immunol. 179:3187. Phan TG, et al. 2007. Nature Immunol. 8:992. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed Wen T, et al. 2013. PNAS. 110:6067. PubMed Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed Product Citations: Galbas T, et al. 2017. J Immunol. 198(2):852-861. PubMed Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed Zhang S, et al. 2017. Nature.. 10.1038/nature24283. PubMed Matsuba S, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01538. PubMed Meers GK, et al. 2018. PLoS One. 13:e0190846. PubMed Oyarce K, et al. 2018. Front Immunol. 9:112. PubMed Nakano-Yokomizo T, et al. 2011. J Exp Med. 208:1661. PubMed Ma J, et al. 2012. J Immunol. 188:5337. PubMed Structure:Protein tyrosine phosphatase (PTP) family, 180-240 kD Distribution: All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice Function:Phosphatase, T and B cell activation Ligand/Receptor:Galectin-1, CD2, CD3, CD4 Biology Area:Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers Molecular Family:CD Molecules Antigen References: 1. Suzuki K, et al. 2000. Immunity 13:691.