EGF (Epithelial Growth Factor), human recombinant Catalog CB031-0043
Description The human Epithelial Growth Factor (EGF) is a growth factor important to proliferation of many types of cells, including stem cells.ExCell produces the full-length EGF in Escherichia coli as a single chain,
non-glycosylated, polypeptide with an apparent molecular mass of 6 kDa..
Source Escherichia coli. Endotoxin level of this product is lower than 0.1 EU/ug.
Formulation Lyophilized from sterile filtered PBS solution, carrier-free.
Reconstitution Before reconstitution, we recommend a brief spin to drive down any material dislodged from the bottom of the tube. It is recommended to reconstitute the lyophilized product in sterile water to no less than 100ug/ml. Further dilutions should be made in sterile buffer or medium containing carrier proteins, such as albumin or serum.
Stability The lyophilized protein is stable for at least 1 year if stored at -80 degree C.Reconstituted protein is stable for at least 2 week at 4 degree C, but should be stored in aliquots at -80 degree C for longer term. Avoid repeated freeze/thaw.
Purity Greater than 97% by SDS-PAGE analysis
Biological Activity The activity was determined by a cell proliferation assay of mouse fibroblasts. The EC50 ranges from 0.1 – 1.0 ng/ml.
For Research Use Only. Not for Use in Humans.