- ￥1260 - 3150
- Leading Biology
- AMR13114N
- 美国
- 2025年07月12日
- WB (Arabidopsis thaliana, Mus musculus, Homo sapiens, Other, Oreochroms mossambcus) IP (Arabidopsis thaliana, Homo sapiens, Mus musculus, Other) IHC (Mus musculus) IF (Mus musculus) ChIP (Arabidopsis thaliana)
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 靶点：
DDDDK; DDDDK tag; DDDDK-tag
- 浓度：
Affinity purification
- 应用范围：
WB (Arabidopsis thaliana, Mus musculus, Homo sapiens, Other, Oreochroms mossambcus) IP (Arabidopsis thaliana, Homo sapiens, Mus musculus, Other) IHC (Mus musculus) IF (Mus musculus) ChIP (Arabidopsis thaliana)
- 宿主：
Rabbit
- 保质期：
1-2年
- 供应商：
安诺伦（北京）生物科技有限公司
- 保存条件：
Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
- 形态：
形式：Liquid 储存溶液：Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
- 亚型：
IgG
- 免疫原：
A synthetic peptide corresponding to DDDDK tag.
- 规格：
50 µl/100 µl/200 µl
| 规格： | 50 µl | 产品价格： | ￥1260.0 |
|---|---|---|---|
| 规格： | 100 µl | 产品价格： | ￥1890.0 |
| 规格： | 200 µl | 产品价格： | ￥3150.0 |
稀释方法：WB 1:500 - 1:2000
IHC 1:50 - 1:500
IF 1:50 - 1:500
IP 1:50 - 1:500
总结：FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography.A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting.
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文献和实验-His-Pro-Gln-Phe-Glu-Lys) 可放在N- 或 C-terminus 不影响蛋白折叠和功能，无需移除 分子量 1058Da Twin-Strep-tag® （也有叫做双Strep-tag®II） 28 aa 标签(WSHPQFEK-GGGSGGGSGG-SA-WSHPQFEK) 两段Strep-tag®II标签序列 较Strep-tag®II对于Strep-Tactin®有更高亲和力 分子量 2887Da 二者均为小标签，不会对蛋白质的结构和功能活性产生影响，因此通常不需要在纯化
干货 | CUT and Tag 让 ChIP-Seq更简单高效！
实验；其中，pA-Tn5 转座子使用的终浓度为 0.04μM ，N* 公司转座子按照 1:100 进行稀释，一抗 H3K27me3（CST，#9733），二抗为 Goat anti Rabbit（Bioworld，#BS13271）。使用 Agilent 2100 Bioanalyzer 比较 CUT&Tag 文库分布。 图 6. TD902 CUT&Tag 文库峰型图 图 7. N* 公司同类产品 CUT&Tag 文库峰型图 结果显示，Vazyme的pA-Tn5转座子切割活性
mlAdd 40 g ammonium sulfate for every 100 ml Sup. (slowly):800gStir O/N in cold room.Pour into plastic bottles. Spin at 4 ℃, 7,500 rpm for 20 min in JA-10 rotor.Prepare 4L 100 mM Tris/pH7.8 (400 ml of 1 M stock/4 L) or Binding buffer.Discard Sup. Resuspend
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