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- 详细信息
- 文献和实验
- 技术资料
- 靶点:
BAG-3; BIS; CAIR-1; MFM6
- 浓度:
Affinity purification
- 宿主:
Rabbit
- 适应物种:
Human, Mouse
- 保质期:
1-2年
- 供应商:
安诺伦(北京)生物科技有限公司
- 保存条件:
Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
- 形态:
形式:Liquid 储存溶液:Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
- 亚型:
IgG
- 免疫原:
A synthesized peptide derived from human BAG3
- 规格:
50 µl/100 µl/200 µl
| 规格: | 50 µl | 产品价格: | ¥1650.0 |
|---|---|---|---|
| 规格: | 100 µl | 产品价格: | ¥2685.0 |
| 规格: | 200 µl | 产品价格: | ¥4230.0 |
Observed MW: 74KDa
稀释方法:WB 1:500 - 1:2000
IHC 1:50 - 1:200
总结:BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq, Jul 2008]
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文献和实验mlAdd 40 g ammonium sulfate for every 100 ml Sup. (slowly):800gStir O/N in cold room.Pour into plastic bottles. Spin at 4 ℃, 7,500 rpm for 20 min in JA-10 rotor.Prepare 4L 100 mM Tris/pH7.8 (400 ml of 1 M stock/4 L) or Binding buffer.Discard Sup. Resuspend
优化的GlycoWorks HILIC SPE流程用于mAb型样品中N-糖的定量与稳定回收
相关专题 沃特世公司(美国马萨诸塞州米尔福德市) 应用优势: ■GlycoWorks HILIC μElution提取板提供了一种高效的多聚糖纯化装置 ■采用优化的SPE条件定量 并一致性地回收不同范围内的N-糖 ■优化的SPE条件具有出色的方法稳定性 ■可采用2-AB标记的多聚糖标准品确保样品制备和分析 方法的性能 沃特世解决方案:
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
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