Background: The Ras-like small G proteins, RalA and RalB, are important components of Ras signaling pathways, implicated in the initiation and maintenance of tumorigenic transformation, as well as vesicle transport, apoptosis, transcription, cell migration, and cell proliferation.
Immunogen: Recombinant full length protein of active RalB.
Applications: IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)
Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250
Concentration: 1 mg/ml
Host Species: Mouse
Format: Liquid
Clonality: Monoclonal
Isotype: IgG
Purity: Purified from ascites
Preservative: No
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Anti-Ral-GTP monoclonal antibody recognizes active RalA and RalB of vertebrates.
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing