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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 英文名：
α-tubulin mouse monoclonal antibody
- 供应商：
上海博尔森生物科技有限公司
- 保存条件：
-20℃
- 规格：
100μL
α-tubulin mouse monoclonal antibody
Tubulin is one of several members of a small family of globular proteins. The tubulin superfamily includes fivedistinct families, the alpha-, beta-, gamma-, delta-, and epsilon-tubulins. The most common members of thetubulin family are α-tubulin and β-tubulin, the proteins that make up microtubules. Each has a molecular weight ofapproximately 55 KD. Microtubules are assembled from dimers of α- and β-tubulin.
Product Name: α -tubulin Mouse Monoclonal Antibody
Isotype: IgG1
Clone Number: 9F12
Storage Buffer : PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol
Storage instructions:-20°C. Do not aliquot the antibody
Recommended dilutions: WB: 1:5,000-10,000 IP: 1:200 IF: 1:100-200 IHC: 1:200-500
Optimal dilutions should be determined by the end user. Specificity: The α -tubulin antibody can detects endogenousα -tubulin protein
Alternative Names: alpha tubulin 2, B alpha 1, H2 alpha, Hum a tub 1, TUBA1, TUBA1A,Tubulin alpha 1chain
Molecular Weight: 52KD
Form: Liquid
Reactivity: H, R, M, zebra fish
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文献和实验Monoclonal Antibody Production
Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma，在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应，对应于抗原之不同部分可制成各种不同的抗体复合体。然而，因为一个产生抗体的细胞，只能产生一种抗体，所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种，可形成边产生抗体边增殖的 hybridoma，将每个杂种细胞进行克隆（无性繁殖系）培养，则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成，这样得到的就是单克隆抗体。此抗体在测定
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